M02675-2

antibody from Boster Biological Technology

Targeting: CD3E

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

M02675-2

Provider

Boster Biological Technology

Product name

Anti-CD3 epsilon Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for CD3/CD3E detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IP, IHC, WB. Reactive species: Human CD3/CD3E information: Molecular Weight: 23147 MW; Subcellular Localization: Membrane; Single-pass type I membrane protein.

Reactivity

Human

Host

Rabbit

Antibody clone number

EED-3

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of CD3 epsilon expression in Jurkat cell lysate (M02675-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD3E monoclonal antibody (Catalog # M02675-2) overnight at 4

Additional image