Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- 10-7561 - Provider product page
- Provider
- ABEOMICS Inc.
- Product name
- Anti-CD3e (ABM45B3)
- Antibody type
- Monoclonal
- Description
- CD3e (CD3-epsilon), together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The gene for CD3e have been mapped to band q23 of chromosome 11 in humans and consisted of nine exons. Three exons, encoding the junction of leader peptide and mature protein, are extremely small. The CD3e cytoplasmic tail, highly conserved, can be tentatively subdivided into three regions; the N-terminal region contains a basic amino acid cluster, the central region contains a proline-rich sequence, and the C-terminal region contains the ITAM . Among the CD3 subunits, CD3e is the most likely candidate for performing a critical role in the TCR signaling response. CD3e is represented twice in the TCR complex, serving as a component of both the gamma/epsilon and delta/epsilon dimers. In addition, after TCR cross-linking, CD3e and zeta are the predominant tyrosine-phosphorylated TCR subunits.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
A partial length recombinant CD3e p
rotein (amino acids 2-203) was used
as the immunogen for this antibody
.- Isotype
- IgG
- Antibody clone number
- ABM45B3
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeat freez thawing
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Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of CD3e. Anti-CD3e antibody (Clone: ABM45B3) was tested at 0.5 µg/ml on MOLT-4 lysate.
- Protocol
- Protocol
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD3e in human Tonsil tissue using CD3e antibody (Clone: ABM45B3) at 5 µg/ml.
- Protocol
- Protocol