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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [3]
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- Product number
- Ab094419 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- CD3 Mouse mAb
- Antibody type
- Monoclonal
- Description
- Mouse anti Human CD3 Antibody, Monoclonal (HIT3a), could be used for Flow and so on.Application:Flow: < 0.5 _g/100w cellsProtein FunctionThe CD3 complex mediates signal transduction.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- HIT3a
- Vial size
- 100_g,10_g,1mg,50_g
- Concentration
- Lot by Lot
- Storage
- Shipped at 4¡C. Store at 4¡C long term (36 months). Upon delivery aliquot.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- CD3 Mouse mAb (Ab094419) - Flow Cytometry Flow Cytometry analysis of Jurkat cells labelling CD3 (red) with CD3 Mouse mAb (Ab094419) at 0.1 μg/mL for 1 hour at 4°C. AffiniPure Goat anti-Mouse IgG, Fcγ fragment specific (Alexa Fluor® 647) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- CD3 Mouse mAb (Ab094419) - Flow Cytometry Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD3 with CD3 Mouse mAb (Ab094419) conjugated with biotin (right panel) at 0.1 μg/mL for 1 hour at 4°C, compared with mouse IgG (Ab170221) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156250).
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- CD3 Mouse mAb (Ab094419) - Flow Cytometry Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD3 (red) with CD3 Mouse mAb (Ab094419) conjugated with biotin at 0.1 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156250). Blue - Isotype control, mouse IgG (Ab170221) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.
