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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- AM20299AF-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM20299AF-N, RRID:AB_10755222
- Product name
- anti CD3
- Antibody type
- Monoclonal
- Antigen
- Human T3 cells.
- Reactivity
- Human
- Host
- Hamster
- Isotype
- IgG
- Antibody clone number
- HAM25-1352
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of CD3 epsilon expression in Jurkat cells (Lane 1), MOLT4 cells (Lane 2) and HPB-ALL cells (Lane 3) using AM20299AF-N CD3e antibody.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunoprecipitation of CD3 epsilon from Jurkat cells with Hamster IgG (Lane 1) or AM20299AF-N (Lane 2). After immunoprecipitation with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with AM20299AF-N CD3e antibody.
