Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-37819 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal CD8 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Flow cytometry analysis of shows positive staining of CD8a+ mouse TK-1 lymphocytes. Antibody not recommended for Western blot.
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 100ug
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Flow Cytometry: CD8 Antibody (2.43) [NBP2-37819] - Analysis of CD8 (red histogram) on TK-1 mouse T-cell lymphoma cells. Cells were harvested, fixed with 4% formaldehyde, washed with PBS, and incubated with a CD8 monoclonal antibody at a 1:50 dilution or with PBS alone (black histogram) for 1 hour at room temperature. For flow cytometry analysis, 30-minute incubation with DyLight 488 goat anti-rat IgG secondary antibody was performed and 10,000 cells were acquired for each sample.