Explore
Validate
Learn
Immunocytochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP2-37819 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal CD8 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Flow cytometry analysis of shows positive staining of CD8a+ mouse TK-1 lymphocytes. Antibody not recommended for Western blot.
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 100ug
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD8 Antibody (2.43) [NBP2-37819] - Analysis of CD8 (red histogram) on TK-1 mouse T-cell lymphoma cells. Cells were harvested, fixed with 4% formaldehyde, washed with PBS, and incubated with a CD8 monoclonal antibody at a 1:50 dilution or with PBS alone (black histogram) for 1 hour at room temperature. For flow cytometry analysis, 30-minute incubation with DyLight 488 goat anti-rat IgG secondary antibody was performed and 10,000 cells were acquired for each sample.
