Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- ABIN2507886 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-CD8a Molecule (CD8A) antibody (PE-Cy7)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- RPA-T8
- Vial size
- 100 tests
- Storage
- 2-8°C protected from light
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Supportive validation
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- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: 1, 2. Anti-Excitatory Amino Acid Transporter 1 (extracellular) antibody (ABIN2511068) (1:500). 3, 4. Anti-Excitatory Amino Acid Transporter 1 (extracellular) antibody, preincubated with the control peptide antigen. Expression of EAAT1 in rat hippocampus Immunohistochemical staining was performed in perfusion-fixed free floating rat brain sections (frozen) using Anti-Excitatory Amino Acid Transporter 1 (extracellular) antibody (ABIN2511068), (1:100). A. EAAT1 staining (green) is particularly intense along the subgranular layer (arrows). B. Staining with mouse anti glial fibrillary acidic protein (red). C. Merged picture confirms presence of densely packed astrocytes along the subgranular layer. DAPI is used as a general cell marker (blue) .
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Image(s): Immunofluorescence