PA5-27924

antibody from Invitrogen Antibodies

Targeting: CFB BF, BFD, H2-Bf

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27924

Provider

Invitrogen Antibodies

Product name

Complement Factor B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: Raji, K562, Rat liver.

Concentration

0.87 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Factor B using 30 µg of A) Raji and B) K562 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Factor B polyclonal antibody (Product # PA5-27924) at a dilution of 1:3000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Factor B in Rat tissue extracts (50 µg). Samples was separated by 7.5% SDS-PAGE and the membrane was probed with Factor B, Internal Polyclonal antibody (Product # PA5-27924) at a dilution of 1:3000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Complement Factor B Polyclonal Antibody (Product # PA5-27924). Rat plasma (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Complement Factor B Polyclonal Antibody (Product # PA5-27924) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human hepatoma, using Factor B (Product # PA5-27924) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.