NBP1-97413

antibody from Novus Biologicals

Targeting: GCG GLP-1, GLP1, GLP2, GRPP

Western blot (Data presented on provider website)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

NBP1-97413

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-97413, RRID:AB_11141810

Product name

Mouse Monoclonal GLP1 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified. Reacts with all forms of GLP-1, including precursor and GLP-1(9-37)/GLP-1(9-36amide) metabolite.

Reactivity

Human

Host

Mouse

Antigen sequence

Mid-molecular epitope of GLP-1


Isotype

IgG

Vial size

100 ug

Concentration

1 mg/ml

Storage

Store at 4C in the dark.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: Glucagon Antibody (4F3) - BSA Free [NBP1-97413] - This uses NBP2-23506 as capture antibody in combination with biotinylated detection antibody NBP1-97413 to measure the sum of GLP-1(7-36)amide, GLP-1(1-36)amide and GLP-1(9-36)amide in biological samples. Relative to measuring the two active forms of GLP-1, the responses are reduced by not measuring the contribution of GLP-1(7- 37) to the overall response, but this is compensated for by measuring an approximately equal contribution from GLP-1(1-36)amide. However, the GLP-1(9-36)amide degradation product is also measured, augmenting the response above that of the active forms alone. This assay cannot detect changes in active GLP-1 in response to treatment with DPP-4 inhibitors, and is therefore only suitable for experiments in which changes in GLP-1 degradation are irrelevant.