Antibody data
- Antibody Data
- Antigen structure
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- Validations
- ELISA [1]
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Validation data
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- Product number
- NBP1-97413 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-97413, RRID:AB_11141810
- Product name
- Mouse Monoclonal GLP1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified. Reacts with all forms of GLP-1, including precursor and GLP-1(9-37)/GLP-1(9-36amide) metabolite.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
Mid-molecular epitope of GLP-1
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- 1 mg/ml
- Storage
- Store at 4C in the dark.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- ELISA: Glucagon Antibody (4F3) - BSA Free [NBP1-97413] - This uses NBP2-23506 as capture antibody in combination with biotinylated detection antibody NBP1-97413 to measure the sum of GLP-1(7-36)amide, GLP-1(1-36)amide and GLP-1(9-36)amide in biological samples. Relative to measuring the two active forms of GLP-1, the responses are reduced by not measuring the contribution of GLP-1(7- 37) to the overall response, but this is compensated for by measuring an approximately equal contribution from GLP-1(1-36)amide. However, the GLP-1(9-36)amide degradation product is also measured, augmenting the response above that of the active forms alone. This assay cannot detect changes in active GLP-1 in response to treatment with DPP-4 inhibitors, and is therefore only suitable for experiments in which changes in GLP-1 degradation are irrelevant.