PB9705

antibody from Boster Biological Technology

Targeting: GCG GLP-1, GLP1, GLP2, GRPP

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PB9705

Provider

Boster Biological Technology

Product name

Anti-GLP1/GCG Antibody Picoband™

Antibody type

Polyclonal

Description

Polyclonal antibody for Glucagon/GCG detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P, IF. Reactive species: Human. Glucagon/GCG information: Molecular Weight: 20909 MW; Subcellular Localization: Secreted; Tissue Specificity: Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP-1 and GLP-2 are also secreted in selected neurons in the brain.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

Generation and Biological Characteristics of a Mouse Model of Breast Cancer that Copresents with Diabetes Mellitus.

Li M, Cao J, He Y, Zhou Z, He X, Zhang QQ, Wang LJ, Qi CL
Anatomical record (Hoboken, N.J. : 2007) 2019 Feb;302(2):269-277

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Glutathione prevents chronic oscillating glucose intake-induced β-cell dedifferentiation and failure.

Zhang J, An H, Ni K, Chen B, Li H, Li Y, Sheng G, Zhou C, Xie M, Chen S, Zhou T, Yang G, Chen X, Wu G, Jin S, Li M
Cell death & disease 2019 Apr 11;10(4):321

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Effect of acute exposure to PFOA on mouse liver cells in vivo and in vitro.

Wu X, Liang M, Yang Z, Su M, Yang B
Environmental science and pollution research international 2017 Nov;24(31):24201-24206

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The anti-hyperglycemic efficacy of a lipid-lowering drug Daming capsule and the underlying signaling mechanisms in a rat model of diabetes mellitus.

Zhang Y, Li X, Li J, Zhang Q, Chen X, Liu X, Zhang Y, Zhang H, Yang H, Hu Y, Wu X, Li X, Ju J, Yang B
Scientific reports 2016 Oct 10;6:34284

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A refined-JinQi-JiangTang tablet ameliorates prediabetes by reducing insulin resistance and improving beta cell function in mice.

Gao LH, Liu Q, Liu SN, Chen ZY, Li CN, Lei L, Sun SJ, Li LY, Liu JL, Shen ZF
Journal of ethnopharmacology 2014;151(1):675-85

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Effect of catch-up growth after food restriction on the entero-insular axis in rats.

Chen LL, Yang WH, Zheng J, Hu X, Kong W, Zhang HH
Nutrition & metabolism 2010 May 26;7:45

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of GLP1 using anti-GLP1 antibody (PB9705). GLP1 was detected in paraffin-embedded section of human pancreatic cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GLP1 Antibody (PB9705) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image