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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [2]
- Other assay [2]
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- Product number
- PA5-13442 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Glucagon Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 1.8 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references CD4(+) T cells regulate glucose homeostasis independent of adipose tissue dysfunction in mice.
The Protective Role of Prenatal Alpha Lipoic Acid Supplementation against Pancreatic Oxidative Damage in Offspring of Valproic Acid-Treated Rats: Histological and Molecular Study.
Influence of Cannabinoid Receptor Deficiency on Parameters Involved in Blood Glucose Regulation in Mice.
Brinker G, Froeba J, Arndt L, Braune J, Hobusch C, Lindhorst A, Bechmann I, Gericke M
European journal of immunology 2021 Jun;51(6):1399-1411
European journal of immunology 2021 Jun;51(6):1399-1411
The Protective Role of Prenatal Alpha Lipoic Acid Supplementation against Pancreatic Oxidative Damage in Offspring of Valproic Acid-Treated Rats: Histological and Molecular Study.
Ghoneim FM, Alrefai H, Elsamanoudy AZ, Abo El-Khair SM, Khalaf HA
Biology 2020 Aug 20;9(9)
Biology 2020 Aug 20;9(9)
Influence of Cannabinoid Receptor Deficiency on Parameters Involved in Blood Glucose Regulation in Mice.
Zibolka J, Wolf A, Rieger L, Rothgänger C, Jörns A, Lutz B, Zimmer A, Dehghani F, Bazwinsky-Wutschke I
International journal of molecular sciences 2020 Apr 30;21(9)
International journal of molecular sciences 2020 Apr 30;21(9)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Glucagon in formalin-fixed and paraffin-embedded human hepatocarcinoma. Samples were incubated with Glucagon polyclonal antibody (Product # PA5-13442) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of HepG2 cells using a GCG polyclonal antibody (Product # PA5-13442) (bottom), compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Glucagon in HepG2 cells (bottom histogram). Samples were incubated with Glucagon polyclonal antibody (Product # PA5-13442) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (top histogram).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Western blot analysis of insulin ( a ), glucagon ( b ) and somatostatin ( c ) protein in pancreatic tissue of male and female wild-type (Wt) mice and cannabinoid receptor knockout mouse lines (CB 1 -/- , CB 2 -/- ). ( a ) Insulin protein was decreased in the pancreatic tissue of CB 2 -/- mice. ( b ) Glucagon and ( c ) Somatostatin protein content displayed no significant differences, although a nonsignificant increase in CB 1 -/- was observed. Values are presented as mean (+-S.E.M.) with n = 8-16 mice per group. Each protein level of male and female Wt mice was defined as 100%. *** p < 0.001; unpaired t -test.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Photomicrographs of immunohistochemically stained pancreatic sections. Control sections ( a , b ). ( a ) An anti-insulin immune-reactivity of pancreatic islet showing brown-stained pancreatic beta cells with strong positive immune-reaction (arrowheads). ( b ) An anti-glucagon immune-reactivity of pancreatic islet showing brown-stained pancreatic alpha cells with mild positive immune-reaction (arrow). VPA treated pancreatic sections ( c , d ). ( c ) An anti-insulin immune-reactivity of pancreatic islet showing brown-stained pancreatic beta cells with mild positive immune-reaction (arrowheads). ( d ) An anti-glucagon immune-reactivity of pancreatic islet showing brown-stained pancreatic alpha cells with strong positive immune-reaction (arrow). VPA and ALA treated pancreatic sections ( e , f ). ( e ) An anti-insulin immune-reactivity of pancreatic islet showing brown-stained pancreatic beta cells with strong positive immune-reaction (arrowheads). ( f ) An anti-glucagon immune-reactivity of pancreatic islet showing brown-stained pancreatic alpha-cells with mild positive immune-reaction (arrow).
