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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-120467 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UGT1A9 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive test controls include: A-549, HT-29, MCF7, HeLa, Mouse liver. The target is usually found in the following locations: Endoplasmic reticulum membrane, Microsome, Single-pass membrane protein. Immunogen sequence: GKLLVVPMDG SHWFTMRSVV EKLILRGHEV VVVMPEVSWQ LGRSLNCTVK TYSTSYTLED LDREFKAFAH AQWKAQVRSI YSLLMGSYND IFDLFFSNCR SLFKDKKLVE YLKESSFDAV FLDPFDNCGL IVAKYFSLPS VVFARGILCH YLEEGAQCPA PLSYVPRILL GFSDAMTFKE RVRNHIMHLE EHLLCHRFFK NALEIASEIL QTPVTEYDLY SHTSI
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.56 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of UGT1A9 in various cell lines using a UGT1A9 Polyclonal antibody (Product # PA5-120467) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of UGT1A9 in MCF-7 cells using a UGT1A9 Polyclonal antibody (Product # PA5-120467). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of UGT1A9 in human liver cancer using a Polyclonal antibody (Product # PA5-120467) at a dilution of 1:200.
