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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NB110-3638 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB110-3638, RRID:AB_787958
- Product name
- Mouse Monoclonal Alkaline Phosphatase/ALPP Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Alkaline Phosphatase, Placental - both Regan and Nagao isoenzymes. No cross reactivity with other isoenzymes of Alkaline Phosphatase.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Astrocyte progenitor transplantation promotes regeneration of bulbospinal respiratory axons, recovery of diaphragm function, and a reduced macrophage response following cervical spinal cord injury.
Lipid-lowering effects of Coffea arabica pulp aqueous extract in Caco-2 cells and hypercholesterolemic rats.
Neural Progenitor Cells Promote Axonal Growth and Alter Axonal mRNA Localization in Adult Neurons.
The effect of sterol structure upon clathrin-mediated and clathrin-independent endocytosis.
Methods for siRNA-mediated reduction of mRNA and protein expression in human placental explants, isolated primary cells and cell lines.
Sphingosine-1-phosphate inhibition of placental trophoblast differentiation through a G(i)-coupled receptor response.
Placental alkaline phosphatase expression at the apical and basal plasma membrane in term villous trophoblasts.
Improved gene transfer into baboon marrow repopulating cells using recombinant human fibronectin fragment CH-296 in combination with interleukin-6, stem cell factor, FLT-3 ligand, and megakaryocyte growth and development factor.
Shedding of the lymphocyte L-selectin adhesion molecule is inhibited by a hydroxamic acid-based protease inhibitor. Identification with an L-selectin-alkaline phosphatase reporter.
Shedding of the lymphocyte L-selectin adhesion molecule is inhibited by a hydroxamic acid-based protease inhibitor. Identification with an L-selectin-alkaline phosphatase reporter.
HIV infection of placental macrophages: their potential role in vertical transmission.
HIV infection of placental macrophages: their potential role in vertical transmission.
Non-amyloidogenic cleavage of the beta-amyloid precursor protein by an integral membrane metalloendopeptidase.
Goulão M, Ghosh B, Urban MW, Sahu M, Mercogliano C, Charsar BA, Komaravolu S, Block CG, Smith GM, Wright MC, Lepore AC
Glia 2019 Mar;67(3):452-466
Glia 2019 Mar;67(3):452-466
Lipid-lowering effects of Coffea arabica pulp aqueous extract in Caco-2 cells and hypercholesterolemic rats.
Ontawong A, Duangjai A, Muanprasat C, Pasachan T, Pongchaidecha A, Amornlerdpison D, Srimaroeng C
Phytomedicine : international journal of phytotherapy and phytopharmacology 2019 Jan;52:187-197
Phytomedicine : international journal of phytotherapy and phytopharmacology 2019 Jan;52:187-197
Neural Progenitor Cells Promote Axonal Growth and Alter Axonal mRNA Localization in Adult Neurons.
Merianda TT, Jin Y, Kalinski AL, Sahoo PK, Fischer I, Twiss JL
eNeuro 2017 Jan-Feb;4(1)
eNeuro 2017 Jan-Feb;4(1)
The effect of sterol structure upon clathrin-mediated and clathrin-independent endocytosis.
Kim JH, Singh A, Del Poeta M, Brown DA, London E
Journal of cell science 2017 Aug 15;130(16):2682-2695
Journal of cell science 2017 Aug 15;130(16):2682-2695
Methods for siRNA-mediated reduction of mRNA and protein expression in human placental explants, isolated primary cells and cell lines.
Forbes K, Desforges M, Garside R, Aplin JD, Westwood M
Placenta 2009 Feb;30(2):124-9
Placenta 2009 Feb;30(2):124-9
Sphingosine-1-phosphate inhibition of placental trophoblast differentiation through a G(i)-coupled receptor response.
Johnstone ED, Chan G, Sibley CP, Davidge ST, Lowen B, Guilbert LJ
Journal of lipid research 2005 Sep;46(9):1833-9
Journal of lipid research 2005 Sep;46(9):1833-9
Placental alkaline phosphatase expression at the apical and basal plasma membrane in term villous trophoblasts.
Leitner K, Szlauer R, Ellinger I, Ellinger A, Zimmer KP, Fuchs R
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2001 Sep;49(9):1155-64
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2001 Sep;49(9):1155-64
Improved gene transfer into baboon marrow repopulating cells using recombinant human fibronectin fragment CH-296 in combination with interleukin-6, stem cell factor, FLT-3 ligand, and megakaryocyte growth and development factor.
Kiem HP, Andrews RG, Morris J, Peterson L, Heyward S, Allen JM, Rasko JE, Potter J, Miller AD
Blood 1998 Sep 15;92(6):1878-86
Blood 1998 Sep 15;92(6):1878-86
Shedding of the lymphocyte L-selectin adhesion molecule is inhibited by a hydroxamic acid-based protease inhibitor. Identification with an L-selectin-alkaline phosphatase reporter.
Feehan C, Darlak K, Kahn J, Walcheck B, Spatola AF, Kishimoto TK
The Journal of biological chemistry 1996 Mar 22;271(12):7019-24
The Journal of biological chemistry 1996 Mar 22;271(12):7019-24
Shedding of the lymphocyte L-selectin adhesion molecule is inhibited by a hydroxamic acid-based protease inhibitor. Identification with an L-selectin-alkaline phosphatase reporter.
Feehan C, Darlak K, Kahn J, Walcheck B, Spatola AF, Kishimoto TK
The Journal of biological chemistry 1996 Mar 22;271(12):7019-24
The Journal of biological chemistry 1996 Mar 22;271(12):7019-24
HIV infection of placental macrophages: their potential role in vertical transmission.
Kesson AM, Fear WR, Williams L, Chang J, King NJ, Cunningham AL
Journal of leukocyte biology 1994 Sep;56(3):241-6
Journal of leukocyte biology 1994 Sep;56(3):241-6
HIV infection of placental macrophages: their potential role in vertical transmission.
Kesson AM, Fear WR, Williams L, Chang J, King NJ, Cunningham AL
Journal of leukocyte biology 1994 Sep;56(3):241-6
Journal of leukocyte biology 1994 Sep;56(3):241-6
Non-amyloidogenic cleavage of the beta-amyloid precursor protein by an integral membrane metalloendopeptidase.
Roberts SB, Ripellino JA, Ingalls KM, Robakis NK, Felsenstein KM
The Journal of biological chemistry 1994 Jan 28;269(4):3111-6
The Journal of biological chemistry 1994 Jan 28;269(4):3111-6
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Alkaline Phosphatase, Placental Antibody (8B6) [NB110-3638] - Analysis of Alkaline Phosphatase (Placental) expression in JAR whole cell lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Alkaline Phosphatase, Placental Antibody (8B6) [NB110-3638] - IHC staining of Alkaline Phosphatase (Placental) in human placenta using DAB with hematoxylin counterstain. Proteolytic Induced Epitope Retrieval (PIER) was used.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - Detection of Alkaline Phosphatase/ALPP in Human HeLa Cell Line by Flow Cytometry. Human HeLa cell line was stained with Mouse Anti- Alkaline Phosphatase/ALPP Monoclonal Antibody (Catalog # NB110-3638, filled histogram), or Mouse IgG2A isotype control (Catalog # MAB003, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
