Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-30311 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Placental Alkaline Phosphatase Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, U87-MG.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Placental Alkaline Phosphatase Polyclonal Antibody (Product # PA5-30311). Sample (30 µg of whole cell lysate). Lane A: NT2D1. Lane B: U87-MG . 7.5% SDS PAGE. Placental Alkaline Phosphatase Polyclonal Antibody (Product # PA5-30311) diluted at 1:3,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Placental Alkaline Phosphatase Polyclonal Antibody immunoprecipitates PLAP protein in IP experiments. IP Sample: NT2D1 whole cell lysate/extract A : Control with 3 µg of pre-immune rabbit IgG B : Immunoprecipitation of PLAP by 3 µg of Placental Alkaline Phosphatase Polyclonal Antibody (Product # PA5-30311) 7.5% SDS-PAGE The immunoprecipitated PLAP protein was detected by Placental Alkaline Phosphatase Polyclonal Antibody (Product # PA5-30311) diluted at 1:1,000. Anti-rabbit IgG (HRP) was used as a secondary reagent.