LS-C407795

antibody from LSBio

Targeting: EIF6 b(2)gcn, EIF3A, ITGB4BP, p27BBP

Antibody data

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Validation data

Product number: LS-C407795 - Provider product page
Provider: LSBio
Product name: EIF6 Antibody (aa66-210) LS-C407795
Antibody type: Polyclonal
Description: Immunogen affinity purified
Reactivity: Human, Mouse, Rat, Canine
Host: Rabbit
Storage: At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

EIF6 protein structure - LS-C407795 shown in red.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: EIF6 antibody IHC-paraffin. IHC(P): Mouse Intestine Tissue.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: EIF6 antibody IHC-paraffin. IHC(P): Rat Testis Tissue.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: EIF6 antibody IHC-paraffin. IHC(P): Human Intestinal Cancer Tissue.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: IHC analysis of EIF6 using anti-EIF6 antibody. EIF6 was detected in immunocytochemical section of SMMC-7721 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-EIF6 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: IHC analysis of EIF6 using anti-EIF6 antibody. EIF6 was detected in immunocytochemical section of SW480 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-EIF6 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.