Antibody data
- Antibody Data
- Antigen structure
- References [26]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- 11475-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#11475-1-AP, RRID:AB_2147094
- Product name
- MTAP antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated MTAP antibody (Cat. #11475-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, rat, mouse and has been validated for the following applications: IF, IHC, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Clinicopathological characterisation of MTAP alterations in gastrointestinal cancers.
JAZF1: A Metabolic Regulator of Sensitivity to a Polyamine-Targeted Therapy.
Integrative Clinical and Genomic Characterization of MTAP-deficient Metastatic Urothelial Cancer.
MTAP deficiency creates an exploitable target for antifolate therapy in 9p21-loss cancers.
Polyamine pathway activity promotes cysteine essentiality in cancer cells.
Pharmacological polyamine catabolism upregulation with methionine salvage pathway inhibition as an effective prostate cancer therapy.
Loss of 5'-Methylthioadenosine Phosphorylase (MTAP) is Frequent in High-Grade Gliomas; Nevertheless, it is Not Associated with Higher Tumor Aggressiveness.
HSP60-regulated Mitochondrial Proteostasis and Protein Translation Promote Tumor Growth of Ovarian Cancer.
Abstracts from USCAP 2019: Pulmonary Pathology (1803-1896).
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2019 Mar;32(Suppl 2):1-76
USCAP 2018 Abstracts: Bone and Soft Tissue Pathology (45-120).
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2018 Mar;98(S1):18-41
CDKN2A loss and PIK3CA mutation in myoepithelial-like metaplastic breast cancer.
Loss of MTAP expression is a negative prognostic marker in Ewing sarcoma family of tumors.
SHARPIN-mediated regulation of protein arginine methyltransferase 5 controls melanoma growth.
Dietary folate levels alter the kinetics and molecular mechanism of prostate cancer recurrence in the CWR22 model.
The essential role of methylthioadenosine phosphorylase in prostate cancer.
Suppressive effects of tumor cell-derived 5'-deoxy-5'-methylthioadenosine on human T cells.
Expression of Methylthioadenosine Phosphorylase (MTAP) in Pilocytic Astrocytomas.
Characterization and Prognostic Significance of Methylthioadenosine Phosphorylase Deficiency in Nasopharyngeal Carcinoma.
Downregulated MTAP expression in myxofibrosarcoma: A characterization of inactivating mechanisms, tumor suppressive function, and therapeutic relevance.
Integrated omic analysis of oropharyngeal carcinomas reveals human papillomavirus (HPV)-dependent regulation of the activator protein 1 (AP-1) pathway.
Ocular expression and distribution of products of the POAG-associated chromosome 9p21 gene region.
Significantly decreased methylthioadenosine phosphorylase expression in malignant melanoma.
The diagnostic value of immunohistochemically detected methylthioadenosine phosphorylase deficiency in malignant pleural mesotheliomas.
Downregulation of methylthioadenosin phosphorylase by homozygous deletion in gastric carcinoma.
Methylthioadenosine phosphorylase and activated insulin-like growth factor-1 receptor/insulin receptor: potential therapeutic targets in chordoma.
Homozygous deletion of MTAP gene as a poor prognosticator in gastrointestinal stromal tumors.
Mauri G, Patelli G, Roazzi L, Valtorta E, Amatu A, Marrapese G, Bonazzina E, Tosi F, Bencardino K, Ciarlo G, Mariella E, Marsoni S, Bardelli A, Bonoldi E, Sartore-Bianchi A, Siena S
Journal of clinical pathology 2024 Feb 13;
Journal of clinical pathology 2024 Feb 13;
JAZF1: A Metabolic Regulator of Sensitivity to a Polyamine-Targeted Therapy.
Rosario SR, Jacobi JJ, Long MD, Affronti HC, Rowsam AM, Smiraglia DJ
Molecular cancer research : MCR 2023 Jan 3;21(1):24-35
Molecular cancer research : MCR 2023 Jan 3;21(1):24-35
Integrative Clinical and Genomic Characterization of MTAP-deficient Metastatic Urothelial Cancer.
Alhalabi O, Zhu Y, Hamza A, Qiao W, Lin Y, Wang RM, Shah AY, Campbell MT, Holla V, Kamat A, Wang WL, Wang J, Chen J, Meng J, Zhang M, Bondaruk J, Titus M, Genovese G, Czerniak BA, Shaw KR, Meric-Bernstam F, Guo CC, Logothetis CJ, Siefker-Radtke A, Msaouel P, Wang L, Liu J, Gao J
European urology oncology 2023 Apr;6(2):228-232
European urology oncology 2023 Apr;6(2):228-232
MTAP deficiency creates an exploitable target for antifolate therapy in 9p21-loss cancers.
Alhalabi O, Chen J, Zhang Y, Lu Y, Wang Q, Ramachandran S, Tidwell RS, Han G, Yan X, Meng J, Wang R, Hoang AG, Wang WL, Song J, Lopez L, Andreev-Drakhlin A, Siefker-Radtke A, Zhang X, Benedict WF, Shah AY, Wang J, Msaouel P, Zhang M, Guo CC, Czerniak B, Behrens C, Soto L, Papadimitrakopoulou V, Lewis J, Rinsurongkawong W, Rinsurongkawong V, Lee J, Roth J, Swisher S, Wistuba I, Heymach J, Wang J, Campbell MT, Efstathiou E, Titus M, Logothetis CJ, Ho TH, Zhang J, Wang L, Gao J
Nature communications 2022 Apr 4;13(1):1797
Nature communications 2022 Apr 4;13(1):1797
Polyamine pathway activity promotes cysteine essentiality in cancer cells.
Zhang T, Bauer C, Newman AC, Uribe AH, Athineos D, Blyth K, Maddocks ODK
Nature metabolism 2020 Oct;2(10):1062-1076
Nature metabolism 2020 Oct;2(10):1062-1076
Pharmacological polyamine catabolism upregulation with methionine salvage pathway inhibition as an effective prostate cancer therapy.
Affronti HC, Rowsam AM, Pellerite AJ, Rosario SR, Long MD, Jacobi JJ, Bianchi-Smiraglia A, Boerlin CS, Gillard BM, Karasik E, Foster BA, Moser M, Wilton JH, Attwood K, Nikiforov MA, Azabdaftari G, Pili R, Phillips JG, Casero RA Jr, Smiraglia DJ
Nature communications 2020 Jan 7;11(1):52
Nature communications 2020 Jan 7;11(1):52
Loss of 5'-Methylthioadenosine Phosphorylase (MTAP) is Frequent in High-Grade Gliomas; Nevertheless, it is Not Associated with Higher Tumor Aggressiveness.
Menezes WP, Silva VAO, Gomes INF, Rosa MN, Spina MLC, Carloni AC, Alves ALV, Melendez M, Almeida GC, Silva LSD, Clara C, da Cunha IW, Hajj GNM, Jones C, Bidinotto LT, Reis RM
Cells 2020 Feb 20;9(2)
Cells 2020 Feb 20;9(2)
HSP60-regulated Mitochondrial Proteostasis and Protein Translation Promote Tumor Growth of Ovarian Cancer.
Guo J, Li X, Zhang W, Chen Y, Zhu S, Chen L, Xu R, Lv Y, Wu D, Guo M, Liu X, Lu W, Deng H
Scientific reports 2019 Sep 2;9(1):12628
Scientific reports 2019 Sep 2;9(1):12628
Abstracts from USCAP 2019: Pulmonary Pathology (1803-1896).
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2019 Mar;32(Suppl 2):1-76
USCAP 2018 Abstracts: Bone and Soft Tissue Pathology (45-120).
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2018 Mar;98(S1):18-41
CDKN2A loss and PIK3CA mutation in myoepithelial-like metaplastic breast cancer.
Bartels S, van Luttikhuizen JL, Christgen M, Mägel L, Luft A, Hänzelmann S, Lehmann U, Schlegelberger B, Leo F, Steinemann D, Kreipe H
The Journal of pathology 2018 Jul;245(3):373-383
The Journal of pathology 2018 Jul;245(3):373-383
Loss of MTAP expression is a negative prognostic marker in Ewing sarcoma family of tumors.
Abrahao-Machado LF, Antunes B, Filippi RZ, Volc S, Boldrini E, Menezes WP, Reis RM, de Camargo OP
Biomarkers in medicine 2018 Jan;12(1):35-44
Biomarkers in medicine 2018 Jan;12(1):35-44
SHARPIN-mediated regulation of protein arginine methyltransferase 5 controls melanoma growth.
Tamiya H, Kim H, Klymenko O, Kim H, Feng Y, Zhang T, Han JY, Murao A, Snipas SJ, Jilaveanu L, Brown K, Kluger H, Zhang H, Iwai K, Ronai ZA
The Journal of clinical investigation 2018 Jan 2;128(1):517-530
The Journal of clinical investigation 2018 Jan 2;128(1):517-530
Dietary folate levels alter the kinetics and molecular mechanism of prostate cancer recurrence in the CWR22 model.
Affronti HC, Long MD, Rosario SR, Gillard BM, Karasik E, Boerlin CS, Pellerite AJ, Foster BA, Attwood K, Pili R, Wilton JH, Campbell MJ, Smiraglia DJ
Oncotarget 2017 Nov 28;8(61):103758-103774
Oncotarget 2017 Nov 28;8(61):103758-103774
The essential role of methylthioadenosine phosphorylase in prostate cancer.
Bistulfi G, Affronti HC, Foster BA, Karasik E, Gillard B, Morrison C, Mohler J, Phillips JG, Smiraglia DJ
Oncotarget 2016 Mar 22;7(12):14380-93
Oncotarget 2016 Mar 22;7(12):14380-93
Suppressive effects of tumor cell-derived 5'-deoxy-5'-methylthioadenosine on human T cells.
Henrich FC, Singer K, Poller K, Bernhardt L, Strobl CD, Limm K, Ritter AP, Gottfried E, Völkl S, Jacobs B, Peter K, Mougiakakos D, Dettmer K, Oefner PJ, Bosserhoff AK, Kreutz MP, Aigner M, Mackensen A
Oncoimmunology 2016 Aug;5(8):e1184802
Oncoimmunology 2016 Aug;5(8):e1184802
Expression of Methylthioadenosine Phosphorylase (MTAP) in Pilocytic Astrocytomas.
Becker AP, Scapulatempo-Neto C, Menezes WP, Clara C, Machado HR, Oliveira RS, Neder L, Reis RM
Pathobiology : journal of immunopathology, molecular and cellular biology 2015;82(2):84-9
Pathobiology : journal of immunopathology, molecular and cellular biology 2015;82(2):84-9
Characterization and Prognostic Significance of Methylthioadenosine Phosphorylase Deficiency in Nasopharyngeal Carcinoma.
He HL, Lee YE, Shiue YL, Lee SW, Chen TJ, Li CF
Medicine 2015 Dec;94(49):e2271
Medicine 2015 Dec;94(49):e2271
Downregulated MTAP expression in myxofibrosarcoma: A characterization of inactivating mechanisms, tumor suppressive function, and therapeutic relevance.
Li CF, Fang FM, Kung HJ, Chen LT, Wang JW, Tsai JW, Yu SC, Wang YH, Li SH, Huang HY
Oncotarget 2014 Nov 30;5(22):11428-41
Oncotarget 2014 Nov 30;5(22):11428-41
Integrated omic analysis of oropharyngeal carcinomas reveals human papillomavirus (HPV)-dependent regulation of the activator protein 1 (AP-1) pathway.
Sepiashvili L, Waggott D, Hui A, Shi W, Su S, Ignatchenko A, Ignatchenko V, Laureano M, Huang SH, Xu W, Weinreb I, Waldron J, O'Sullivan B, Irish JC, Boutros PC, Liu FF, Kislinger T
Molecular & cellular proteomics : MCP 2014 Dec;13(12):3572-84
Molecular & cellular proteomics : MCP 2014 Dec;13(12):3572-84
Ocular expression and distribution of products of the POAG-associated chromosome 9p21 gene region.
Chidlow G, Wood JP, Sharma S, Dimasi DP, Burdon KP, Casson RJ, Craig JE
PloS one 2013;8(9):e75067
PloS one 2013;8(9):e75067
Significantly decreased methylthioadenosine phosphorylase expression in malignant melanoma.
Gambichler T, Scola N, Bechara FG
The American Journal of dermatopathology 2012 Oct;34(7):777-9
The American Journal of dermatopathology 2012 Oct;34(7):777-9
The diagnostic value of immunohistochemically detected methylthioadenosine phosphorylase deficiency in malignant pleural mesotheliomas.
Zimling ZG, Jørgensen A, Santoni-Rugiu E
Histopathology 2012 May;60(6B):E96-105
Histopathology 2012 May;60(6B):E96-105
Downregulation of methylthioadenosin phosphorylase by homozygous deletion in gastric carcinoma.
Kim J, Kim MA, Min SY, Jee CD, Lee HE, Kim WH
Genes, chromosomes & cancer 2011 Jun;50(6):421-33
Genes, chromosomes & cancer 2011 Jun;50(6):421-33
Methylthioadenosine phosphorylase and activated insulin-like growth factor-1 receptor/insulin receptor: potential therapeutic targets in chordoma.
Sommer J, Itani DM, Homlar KC, Keedy VL, Halpern JL, Holt GE, Schwartz HS, Coffin CM, Kelley MJ, Cates JM
The Journal of pathology 2010 Apr;220(5):608-17
The Journal of pathology 2010 Apr;220(5):608-17
Homozygous deletion of MTAP gene as a poor prognosticator in gastrointestinal stromal tumors.
Huang HY, Li SH, Yu SC, Chou FF, Tzeng CC, Hu TH, Uen YH, Tian YF, Wang YH, Fang FM, Huang WW, Wei YC, Wu JM, Li CF
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Nov 15;15(22):6963-72
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Nov 15;15(22):6963-72
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- MCF7 cells were subjected to SDS PAGE followed by western blot with 11475-1-AP(MTAP antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-MTAP (IP:11475-1-AP, 3ug; Detection:11475-1-AP 1:500) with MCF-7 cells lysate 3250ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The MTAP antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human MTAP. This antibody recognizes human, mouse antigen. The MTAP antibody has been validated for the following applications: ELISA, IHC, IP, WB analysis.