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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [4]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-22000 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MTAP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: A431, HeLa, HepG2, Mouse liver. Predicted reactivity: Mouse (93%), Xenopus laevis (80%), Chicken (83%), Rhesus Monkey (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.18 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MTAP in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min, using a MTAP polyclonal antibody (Product # PA5-22000). Green: Primary antibody at a dilution of 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MTAP Polyclonal Antibody detects MTAP protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MTAP protein stained by MTAP Polyclonal Antibody (Product # PA5-22000) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MTAP Polyclonal Antibody detects MTAP protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MTAP protein stained by MTAP Polyclonal Antibody (Product # PA5-22000) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MTAP Polyclonal Antibody detects MTAP protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MTAP protein stained by MTAP Polyclonal Antibody (Product # PA5-22000) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of MTAP was performed in HeLa whole cell extracts using 5 µg of MTAP Polyclonal Antibody (Product # PA5-22000). Samples were transferred to a membrane and probed with MTAP Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SAS xenograft , using MTAP (Product # PA5-22000) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of MTAP was performed in HeLa whole cell extracts using 5 µg of MTAP Polyclonal Antibody (Product # PA5-22000). Samples were transferred to a membrane and probed with MTAP Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
