Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- IC2574G - Provider product page
- Provider
- R&D Systems
- Product name
- Anti-Human ILT6/CD85e Alexa Fluor 488-conjugated Antigen Affinity-purified Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Mouse myeloma cell line NS0-derived recombinant human ILT6/CD85e, Arg18-Glu439
- Description
- Antigen Affinity-purified
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Green dye
- Antigen sequence
Q8N6C8
- Isotype
- IgG
- Vial size
- 100 Tests
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of LILRA3/CD85e in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Goat Anti-Human LILRA3/CD85e Alexa Fluor® 488-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2574G) or (B) Normal Goat IgG Alexa Fluor 488 Control (Catalog # IC108G). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.