Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
Reference
Comment
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- Product number
- PA1-20991 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-WT1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-20991 detects Wilm's tumor in Human, Mouse and Rat samples. PA1-20991 has been successfully used in Immunohistochemistry (Paraffin) procedures. Predicted molecular weight of 51 kDa. PA1-20991 immunogen corresponds to Synthetic peptide (Human) (C terminal).
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 500 µL
- Concentration
- Lot Dependent
- Storage
- 4° C, do not freeze
Submitted references Semiautomated quantitative image analysis of glomerular immunohistochemistry markers desmin, vimentin, podocin, synaptopodin and WT-1 in acute and chronic rat kidney disease models.
Funk J, Ott V, Herrmann A, Rapp W, Raab S, Riboulet W, Vandjour A, Hainaut E, Benardeau A, Singer T, Jacobsen B
Histochemistry and cell biology 2016 Mar;145(3):315-26
Histochemistry and cell biology 2016 Mar;145(3):315-26
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-WT1 Polyclonal Antibody (Product # PA1-20991) and a 48 kDa band corresponding to WT-1 was observed in across all tested cell lines and tissues along with few additional uncharacterized bands. Whole cell extracts (30 µg lysate) of OVCAR-3 (Lane 1), Embryoid bodies (Lane 2), Mouse heart (Lane 3), Mouse testis (Lane 4), Mouse kidney (Lane 5), Rat kidney (Lane 6), Mouse lung (Lane 7) and Rat lung (Lane 8) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Wilm's Tumor in human Wilm’s tumor using a Wilm's Tumor polyclonal antibody (Product # PA1-20991).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Wilm's Tumor in Wilm's tumour tissue using a Wilm's Tumor polyclonal antibody (Product # PA1-20991).