PA1-20991

antibody from Invitrogen Antibodies

Targeting: WT1 AWT1, GUD, NPHS4, WAGR, WIT-2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Gel shift (Recommended by provider)

Antibody Data

Product number

PA1-20991

Provider

Invitrogen Antibodies

Product name

Anti-WT1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1-20991 detects Wilm's tumor in Human, Mouse and Rat samples. PA1-20991 has been successfully used in Immunohistochemistry (Paraffin) procedures. Predicted molecular weight of 51 kDa. PA1-20991 immunogen corresponds to Synthetic peptide (Human) (C terminal).

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

500 µL

Concentration

Lot Dependent

Storage

4° C, do not freeze

References

Submitted references

Semiautomated quantitative image analysis of glomerular immunohistochemistry markers desmin, vimentin, podocin, synaptopodin and WT-1 in acute and chronic rat kidney disease models.

Funk J, Ott V, Herrmann A, Rapp W, Raab S, Riboulet W, Vandjour A, Hainaut E, Benardeau A, Singer T, Jacobsen B
Histochemistry and cell biology 2016 Mar;145(3):315-26

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-WT1 Polyclonal Antibody (Product # PA1-20991) and a 48 kDa band corresponding to WT-1 was observed in across all tested cell lines and tissues along with few additional uncharacterized bands. Whole cell extracts (30 µg lysate) of OVCAR-3 (Lane 1), Embryoid bodies (Lane 2), Mouse heart (Lane 3), Mouse testis (Lane 4), Mouse kidney (Lane 5), Rat kidney (Lane 6), Mouse lung (Lane 7) and Rat lung (Lane 8) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Wilm's Tumor in human Wilm’s tumor using a Wilm's Tumor polyclonal antibody (Product # PA1-20991).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Wilm's Tumor in Wilm's tumour tissue using a Wilm's Tumor polyclonal antibody (Product # PA1-20991).