Explore
Validate
Learn
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- BAF5824 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat AIF Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. AIF antibodies are ideal for immunocytochemistry colocalization studies in mitochondria. The unconjugated antibody detects human, mouse, and rat AIF in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Sheep
- Conjugate
- Biotin
- Antigen sequence
O95831- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- AIF in HeLa Human Cell Line. AIF was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human/Mouse/Rat AIF Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF5824) at 5 µg/mL overnight at 4 ° C. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (orange; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to mitochondria. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
