TA811193

antibody from OriGene

Targeting: AIFM1 AIF, AUNX1, CMTX4, DFNX5, NAMSD, PDCD8

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA811193

Provider

OriGene

Product name

AIFM1 mouse monoclonal antibody,clone OTI5D2

Antibody type

Monoclonal

Description

AIFM1 mouse monoclonal antibody,clone OTI5D2

Host

Mouse

Conjugate

Unconjugated

Epitope

AIFM1

Isotype

IgG

Antibody clone number

OTI5D2

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AIFM1 (RC225349, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AIFM1.(1:2000)

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 2 different cell lines and mouse liver tissue lysate by using anti-AIFM1 monoclonal antibody .(1:500)

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human adult heart tissue within the normal limits using anti-AIFM1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA811193)(1:500)

Validation comment

IHC