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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Western blot [1]
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- Product number
- MAB5736 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Caveolin-1 Antibody
- Antibody type
- Monoclonal
- Description
- Caveolin-1
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Hyperthermia and associated changes in membrane fluidity potentiate P2X7 activation to promote tumor cell death.
A preliminary study on the interaction between Asn-Gly-Arg (NGR)-modified multifunctional nanoparticles and vascular epithelial cells.
Enterohemorrhagic Escherichia coli hemolysin employs outer membrane vesicles to target mitochondria and cause endothelial and epithelial apoptosis.
Regulation of amino acid/carnitine transporter B 0,+ (ATB 0,+) in astrocytes by protein kinase C: independent effects on raft and non-raft transporter subpopulations.
Immunopurification and characterization of rat adipocyte caveolae suggest their dissociation from insulin signaling.
de Andrade Mello P, Bian S, Savio LEB, Zhang H, Zhang J, Junger W, Wink MR, Lenz G, Buffon A, Wu Y, Robson SC
Oncotarget 2017 Sep 15;8(40):67254-67268
Oncotarget 2017 Sep 15;8(40):67254-67268
A preliminary study on the interaction between Asn-Gly-Arg (NGR)-modified multifunctional nanoparticles and vascular epithelial cells.
Liu C, Liu T, Yu X, Gu Y
Acta pharmaceutica Sinica. B 2017 May;7(3):361-372
Acta pharmaceutica Sinica. B 2017 May;7(3):361-372
Enterohemorrhagic Escherichia coli hemolysin employs outer membrane vesicles to target mitochondria and cause endothelial and epithelial apoptosis.
Bielaszewska M, Rüter C, Kunsmann L, Greune L, Bauwens A, Zhang W, Kuczius T, Kim KS, Mellmann A, Schmidt MA, Karch H
PLoS pathogens 2013;9(12):e1003797
PLoS pathogens 2013;9(12):e1003797
Regulation of amino acid/carnitine transporter B 0,+ (ATB 0,+) in astrocytes by protein kinase C: independent effects on raft and non-raft transporter subpopulations.
Samluk Ł, Czeredys M, Nałęcz KA
Journal of neurochemistry 2010 Dec;115(6):1386-97
Journal of neurochemistry 2010 Dec;115(6):1386-97
Immunopurification and characterization of rat adipocyte caveolae suggest their dissociation from insulin signaling.
Souto RP, Vallega G, Wharton J, Vinten J, Tranum-Jensen J, Pilch PF
The Journal of biological chemistry 2003 May 16;278(20):18321-9
The Journal of biological chemistry 2003 May 16;278(20):18321-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Caveolin-1 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells and HMVEC human microvascular endothelial cells. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Caveolin-1 Monoclonal Antibody (Catalog # MAB5736) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Caveolin-1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
