Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 12-1939-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD193 (CCR3) Monoclonal Antibody (eBio5E8-G9-B4 (5E8-G9-B4)), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBio5E8-G9-B4 monoclonal antibody reacts with human CD193 (CCR3, CKR3). CD193 is a member of the seven transmembrane G-protein coupled receptor (GPCR) family, and is a high affinity chemokine receptor for the chemokines eotaxin-1 (CCL11), eotaxin-2 (CCL24), eotaxin-3 (CCL26) and MCP-4 (CCL13), but has also been reported to bind RANTES, MCP-3 and MCP-4. CD193 is highly expressed on the surface of eosinophils, and is the key eosinophil chemokine receptor responsible for the regulation of eosinophil migration and function. In addition to eosinophils, CD193 is also expressed on CD4+ TH2 cells, basophils, mast cells, mononuclear phagocytes, platelets, CD34+ hematopoietic progenitors and airway epithelial cells. CD193 likely plays an important role in the migration of eosinophils in allergic airway inflammation and asthma. CD193 can also serve as a receptor for HIV-1 cell entry. Applications Reported: This eBio5E8-G9-B4 (5E8-G9-B4) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio5E8-G9-B4 (5E8-G9-B4) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- eBio5E8-G9-B4 (5E8-G9-B4)
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Sensing and responding to allergic response cytokines through a genetically encoded circuit.
In silico epitope prediction, expression and functional analysis of Per a 10 allergen from the American cockroach.
Molecular cloning, expression, IgE binding activities and in silico epitope prediction of Per a 9 allergens of the American cockroach.
Preparation and identification of Per a 5 as a novel American cockroach allergen.
Chassin H, Geering B, Schukur L, Ausländer D, Lang B, Fussenegger M
Nature communications 2017 Oct 24;8(1):1101
Nature communications 2017 Oct 24;8(1):1101
In silico epitope prediction, expression and functional analysis of Per a 10 allergen from the American cockroach.
Tong X, Guo M, Jin M, Chen H, Li Y, Wei JF
International journal of molecular medicine 2016 Dec;38(6):1806-1814
International journal of molecular medicine 2016 Dec;38(6):1806-1814
Molecular cloning, expression, IgE binding activities and in silico epitope prediction of Per a 9 allergens of the American cockroach.
Yang H, Chen H, Jin M, Xie H, He S, Wei JF
International journal of molecular medicine 2016 Dec;38(6):1795-1805
International journal of molecular medicine 2016 Dec;38(6):1795-1805
Preparation and identification of Per a 5 as a novel American cockroach allergen.
Wei JF, Yang H, Li D, Gao P, He S
Mediators of inflammation 2014;2014:591468
Mediators of inflammation 2014;2014:591468
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD16 FITC (Product # 11-0168-42) and Mouse IgG2b K Isotype Control PE (Product # 12-4732-81) (left) or Anti-Human CD193 (CCR3) PE (right). Cells in the granulocyte gate were used for analysis.
- Conjugate
- Yellow dye