711263

antibody from Invitrogen Antibodies

Targeting: CCR3 CC-CKR-3, CD193, CKR3, CMKBR3

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

711263

Provider

Invitrogen Antibodies

Product name

CCR3 Recombinant Polyclonal Antibody (23HCLC)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

23HCLC

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

For immunofluorescence analysis, THP1 cells were fixed and permeabilized for detection of endogenous CCR3 using Anti-CCR3 Recombinant Rabbit Polyclonal Antibody (Product # 711263, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of CCR3 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating membrane localization of CCR3. Panel e)represents control cells with no primary antibody to assess background Panel f) shows no signal in HeLa cells which is negative for CCR3 expression. The images were captured at 60X magnification