Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB3477 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CCR7 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse CCR7.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
NP_031745
- Isotype
- IgG
- Antibody clone number
- 4B12
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references TGF-β1-induced EMT promotes targeted migration of breast cancer cells through the lymphatic system by the activation of CCR7/CCL21-mediated chemotaxis.
FOXO1 regulates dendritic cell activity through ICAM-1 and CCR7.
Deficiency of lymph node-resident dendritic cells (DCs) and dysregulation of DC chemoattractants in a malnourished mouse model of Leishmania donovani infection.
CC-Chemokine receptor CCR7: a key molecule for lymph node metastasis in esophageal squamous cell carcinoma.
Direct B cell stimulation by dendritic cells in a mouse model of lupus.
Pang MF, Georgoudaki AM, Lambut L, Johansson J, Tabor V, Hagikura K, Jin Y, Jansson M, Alexander JS, Nelson CM, Jakobsson L, Betsholtz C, Sund M, Karlsson MC, Fuxe J
Oncogene 2016 Feb 11;35(6):748-60
Oncogene 2016 Feb 11;35(6):748-60
FOXO1 regulates dendritic cell activity through ICAM-1 and CCR7.
Dong G, Wang Y, Xiao W, Pacios Pujado S, Xu F, Tian C, Xiao E, Choi Y, Graves DT
Journal of immunology (Baltimore, Md. : 1950) 2015 Apr 15;194(8):3745-55
Journal of immunology (Baltimore, Md. : 1950) 2015 Apr 15;194(8):3745-55
Deficiency of lymph node-resident dendritic cells (DCs) and dysregulation of DC chemoattractants in a malnourished mouse model of Leishmania donovani infection.
Ibrahim MK, Barnes JL, Osorio EY, Anstead GM, Jimenez F, Osterholzer JJ, Travi BL, Ahuja SS, White AC Jr, Melby PC
Infection and immunity 2014 Aug;82(8):3098-112
Infection and immunity 2014 Aug;82(8):3098-112
CC-Chemokine receptor CCR7: a key molecule for lymph node metastasis in esophageal squamous cell carcinoma.
Irino T, Takeuchi H, Matsuda S, Saikawa Y, Kawakubo H, Wada N, Takahashi T, Nakamura R, Fukuda K, Omori T, Kitagawa Y
BMC cancer 2014 Apr 26;14:291
BMC cancer 2014 Apr 26;14:291
Direct B cell stimulation by dendritic cells in a mouse model of lupus.
Wan S, Zhou Z, Duan B, Morel L
Arthritis and rheumatism 2008 Jun;58(6):1741-50
Arthritis and rheumatism 2008 Jun;58(6):1741-50
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CCR7 in Mouse Splenocytes. CCR7 was detected in immersion fixed non-stimulated mouse splenocytes using 25 µg/mL Rat Anti-Mouse CCR7 Monoclonal Antibody (Catalog # MAB3477) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CCR7 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Rat Anti-Mouse CCR7 Monoclonal Antibody (Catalog # MAB3477) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CCL19/MIP-3 beta and Neutralization by Mouse CCR7 Antibody. Recombinant Mouse CCL19/MIP-3 beta (Catalog # 440-M3) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL19/MIP-3 beta (25 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCR7 Monoclonal Antibody (Catalog # MAB3477). The ND50 is typically 2-10 µg/mL.