Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [2]
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Validation data
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- Product number
- FAB197N-025 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CCR7 Alexa Fluor® 700-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human CCR7.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Near infrared dye
- Antigen sequence
AAA58615
- Isotype
- IgG
- Antibody clone number
- 150503
- Vial size
- 25 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Hypercytotoxicity and rapid loss of NKp44+ innate lymphoid cells during acute SIV infection.
Rhesus macaque lymph node PD-1(hi)CD4+ T cells express high levels of CXCR5 and IL-21 and display a CCR7(lo)ICOS+Bcl6+ T-follicular helper (Tfh) cell phenotype.
Phenotypic complexity of T regulatory subsets in patients with B-chronic lymphocytic leukemia.
Li H, Richert-Spuhler LE, Evans TI, Gillis J, Connole M, Estes JD, Keele BF, Klatt NR, Reeves RK
PLoS pathogens 2014 Dec;10(12):e1004551
PLoS pathogens 2014 Dec;10(12):e1004551
Rhesus macaque lymph node PD-1(hi)CD4+ T cells express high levels of CXCR5 and IL-21 and display a CCR7(lo)ICOS+Bcl6+ T-follicular helper (Tfh) cell phenotype.
Onabajo OO, George J, Lewis MG, Mattapallil JJ
PloS one 2013;8(3):e59758
PloS one 2013;8(3):e59758
Phenotypic complexity of T regulatory subsets in patients with B-chronic lymphocytic leukemia.
Biancotto A, Dagur PK, Fuchs JC, Wiestner A, Bagwell CB, McCoy JP Jr
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2012 Feb;25(2):246-59
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2012 Feb;25(2):246-59
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CCR7 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P) and either (A) Mouse Anti-Human CCR7 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB197N) or (B) Mouse IgG2A Alexa Fluor 700 Isotype Control (Catalog # IC003N). View our protocol for Staining Membrane-associated Proteins.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CCR7 in HEK293 Human Cell Line Transfected with Human CCR7 and eGFP by Flow Cytometry. The specificity of Mouse Anti-Human CCR7 Clone 150503 was demonstrated by its ability to react with (A) HEK293 human embryonic kidney cell line transfected with human CCR7 and not react with (B) irrelevant HEK293 transfectants. Antibody binding was monitored using Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).