Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 13-1979-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD197 (CCR7) Monoclonal Antibody (3D12), Biotin, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 3D12 monoclonal antibody reacts with human CCR7, also known as EBI-1 and CD197. CCR7 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains and functions as a receptor for 6Ckine/SLC (secondary lymphoid-tissue chemokine), CCL19 and CCL21. CCR7 has been shown to be internalized via clathrin-coated pits and the majority recycled back to the plasma membrane. CCR7 is expressed on T cells and can be used to distinguish populations of naive from central and effector memory T cells. CCR7 has been shown to play a role in migration of memory T cells to inflamed tissue. Expression of CCR7 is also found on DC's. During DC maturation CCR7 expression increases and is thought to be involved in a variety of functions: chemotaxis to the lymph node, cellular architecture, rate of endocytosis, survival and maturation. Expression of CCR7 on the cell surface can be down regulated upon ligand binding. Applications Reported: This 3D12 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 3D12 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. It is recommended that the staining incubation time be increased to at least 45 minutes at 2-8°C for optimal staining. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- 3D12
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Role of TLX1 in T-cell acute lymphoblastic leukaemia pathogenesis.
Proliferation and differentiation potential of human CD8+ memory T-cell subsets in response to antigen or homeostatic cytokines.
Two subsets of memory T lymphocytes with distinct homing potentials and effector functions.
Riz I, Hawley TS, Johnston H, Hawley RG
British journal of haematology 2009 Apr;145(1):140-3
British journal of haematology 2009 Apr;145(1):140-3
Proliferation and differentiation potential of human CD8+ memory T-cell subsets in response to antigen or homeostatic cytokines.
Geginat J, Lanzavecchia A, Sallusto F
Blood 2003 Jun 1;101(11):4260-6
Blood 2003 Jun 1;101(11):4260-6
Two subsets of memory T lymphocytes with distinct homing potentials and effector functions.
Sallusto F, Lenig D, Förster R, Lipp M, Lanzavecchia A
Nature 1999 Oct 14;401(6754):708-12
Nature 1999 Oct 14;401(6754):708-12
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD45RA FITC (Product # 11-0458-42) and 1.0 µg of Rat IgG2a K Isotype Control Biotin (Product # 13-4321-82) (left) or 1.0 µg of Anti-Human CD197 (CCR7) Biotin (right) followed by Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Biotin