13-1979-82

antibody from Invitrogen Antibodies
Targeting: CCR7 BLR2, CD197, CDw197, CMKBR7, EBI1
Provider product page for 13-1979-82

Antibody data

Product number
13-1979-82 - Provider product page
Provider
Invitrogen Antibodies
Product name
CD197 (CCR7) Monoclonal Antibody (3D12), Biotin, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The 3D12 monoclonal antibody reacts with human CCR7, also known as EBI-1 and CD197. CCR7 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains and functions as a receptor for 6Ckine/SLC (secondary lymphoid-tissue chemokine), CCL19 and CCL21. CCR7 has been shown to be internalized via clathrin-coated pits and the majority recycled back to the plasma membrane. CCR7 is expressed on T cells and can be used to distinguish populations of naive from central and effector memory T cells. CCR7 has been shown to play a role in migration of memory T cells to inflamed tissue. Expression of CCR7 is also found on DC's. During DC maturation CCR7 expression increases and is thought to be involved in a variety of functions: chemotaxis to the lymph node, cellular architecture, rate of endocytosis, survival and maturation. Expression of CCR7 on the cell surface can be down regulated upon ligand binding. Applications Reported: This 3D12 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 3D12 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. It is recommended that the staining incubation time be increased to at least 45 minutes at 2-8°C for optimal staining. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Rat
Conjugate
Biotin
Isotype
IgG
Antibody clone number
3D12
Vial size
100 µg
Concentration
0.5 mg/mL
Storage
4° C, store in dark, DO NOT FREEZE!
CCR7 protein structure - 13-1979-82 shown in red.