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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [11]
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- Validations
- Flow cytometry [1]
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- Product number
- 46-1979-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD197 (CCR7) Monoclonal Antibody (3D12), PerCP-eFluor™ 710, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 3D12 monoclonal antibody reacts with human CCR7, also known as EBI-1 and CD197. CCR7 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains and functions as a receptor for 6Ckine/SLC (secondary lymphoid-tissue chemokine), CCL19 and CCL21. CCR7 has been shown to be internalized via clathrin-coated pits and the majority recycled back to the plasma membrane. CCR7 is expressed on T cells and can be used to distinguish populations of naive from central and effector memory T cells. CCR7 has been shown to play a role in migration of memory T cells to inflamed tissue. Expression of CCR7 is also found on DC's. During DC maturation CCR7 expression increases and is thought to be involved in a variety of functions: chemotaxis to the lymph node, cellular architecture, rate of endocytosis, survival and maturation. Expression of CCR7 on the cell surface can be down regulated upon ligand binding. Applications Reported: This 3D12 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 3D12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the staining incubation time be increased to at least 45 minutes at 2-8°C for optimal staining. PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- 3D12
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Alteration of the Immune Microenvironment in HBsAg and HBeAg Dual-Positive Pregnant Women Presenting a High HBV Viral Load.
Neuroimmune Consequences of eIF4E Phosphorylation on Chemotherapy-Induced Peripheral Neuropathy.
Gene Transfer in Adeno-Associated Virus Seropositive Rhesus Macaques Following Rapamycin Treatment and Subcutaneous Delivery of AAV6, but Not Retargeted AAV6 Vectors.
Application of the chemokine-chemokine receptor axis increases the tumor-targeted migration ability of cytokine-induced killer cells in patients with colorectal cancer.
Biochanin a Enhances the Defense Against Salmonella enterica Infection Through AMPK/ULK1/mTOR-Mediated Autophagy and Extracellular Traps and Reversing SPI-1-Dependent Macrophage (MΦ) M2 Polarization.
Oral Supplementation with Baker's Yeast Beta Glucan Is Associated with Altered Monocytes, T Cells and Cytokines following a Bout of Strenuous Exercise.
Phenotypic characterization of regulatory T cells from antiretroviral-naive HIV-1-infected people.
Memory T cells in latent Mycobacterium tuberculosis infection are directed against three antigenic islands and largely contained in a CXCR3+CCR6+ Th1 subset.
Role of TLX1 in T-cell acute lymphoblastic leukaemia pathogenesis.
Proliferation and differentiation potential of human CD8+ memory T-cell subsets in response to antigen or homeostatic cytokines.
Two subsets of memory T lymphocytes with distinct homing potentials and effector functions.
Gao F, Wang H, Li X, Guo F, Yuan Y, Wang X, Zhang Y, Bai G
Journal of inflammation research 2021;14:5619-5632
Journal of inflammation research 2021;14:5619-5632
Neuroimmune Consequences of eIF4E Phosphorylation on Chemotherapy-Induced Peripheral Neuropathy.
Agalave NM, Mody PH, Szabo-Pardi TA, Jeong HS, Burton MD
Frontiers in immunology 2021;12:642420
Frontiers in immunology 2021;12:642420
Gene Transfer in Adeno-Associated Virus Seropositive Rhesus Macaques Following Rapamycin Treatment and Subcutaneous Delivery of AAV6, but Not Retargeted AAV6 Vectors.
Stone D, Kenkel EJ, Loprieno MA, Tanaka M, De Silva Feelixge HS, Kumar AJ, Stensland L, Obenza WM, Wangari S, Ahrens CY, Murnane RD, Peterson CW, Kiem HP, Huang ML, Aubert M, Hu SL, Jerome KR
Human gene therapy 2021 Jan;32(1-2):96-112
Human gene therapy 2021 Jan;32(1-2):96-112
Application of the chemokine-chemokine receptor axis increases the tumor-targeted migration ability of cytokine-induced killer cells in patients with colorectal cancer.
Zou Y, Liang J, Li D, Fang J, Wang L, Wang J, Zhang J, Guo Q, Yan X, Tang H
Oncology letters 2020 Jul;20(1):123-134
Oncology letters 2020 Jul;20(1):123-134
Biochanin a Enhances the Defense Against Salmonella enterica Infection Through AMPK/ULK1/mTOR-Mediated Autophagy and Extracellular Traps and Reversing SPI-1-Dependent Macrophage (MΦ) M2 Polarization.
Zhao X, Tang X, Guo N, An Y, Chen X, Shi C, Wang C, Li Y, Li S, Xu H, Liu M, Wang Y, Yu L
Frontiers in cellular and infection microbiology 2018;8:318
Frontiers in cellular and infection microbiology 2018;8:318
Oral Supplementation with Baker's Yeast Beta Glucan Is Associated with Altered Monocytes, T Cells and Cytokines following a Bout of Strenuous Exercise.
McFarlin BK, Venable AS, Carpenter KC, Henning AL, Ogenstad S
Frontiers in physiology 2017;8:786
Frontiers in physiology 2017;8:786
Phenotypic characterization of regulatory T cells from antiretroviral-naive HIV-1-infected people.
Ambada GN, Ntsama CE, Nji NN, Ngu LN, Sake CN, Lissom A, Tchouangeu FT, Tchadji J, Sosso M, Etoa FX, Nchinda GW
Immunology 2017 Aug;151(4):405-416
Immunology 2017 Aug;151(4):405-416
Memory T cells in latent Mycobacterium tuberculosis infection are directed against three antigenic islands and largely contained in a CXCR3+CCR6+ Th1 subset.
Lindestam Arlehamn CS, Gerasimova A, Mele F, Henderson R, Swann J, Greenbaum JA, Kim Y, Sidney J, James EA, Taplitz R, McKinney DM, Kwok WW, Grey H, Sallusto F, Peters B, Sette A
PLoS pathogens 2013 Jan;9(1):e1003130
PLoS pathogens 2013 Jan;9(1):e1003130
Role of TLX1 in T-cell acute lymphoblastic leukaemia pathogenesis.
Riz I, Hawley TS, Johnston H, Hawley RG
British journal of haematology 2009 Apr;145(1):140-3
British journal of haematology 2009 Apr;145(1):140-3
Proliferation and differentiation potential of human CD8+ memory T-cell subsets in response to antigen or homeostatic cytokines.
Geginat J, Lanzavecchia A, Sallusto F
Blood 2003 Jun 1;101(11):4260-6
Blood 2003 Jun 1;101(11):4260-6
Two subsets of memory T lymphocytes with distinct homing potentials and effector functions.
Sallusto F, Lenig D, Förster R, Lipp M, Lanzavecchia A
Nature 1999 Oct 14;401(6754):708-12
Nature 1999 Oct 14;401(6754):708-12
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD45RA APC (Product # 17-0458-42) and Rat IgG2a K Isotype Control PerCP-eFluor® 710 (Product # 46-4321-82) (left) or Anti-Human CD197 (CCR7) PerCP-eFluor® 710 (right). Cells in the lymphocyte gate were used for analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3. Expression levels of chemokine receptors on CIK cells generated from patients with CRC and healthy donors. (A) Analysis of the expression levels of CCR4, CCR5, CCR7, CXCR3 and CXCR4 on CIK cells via flow cytometry revealed that CXCR3 and CXCR4 had higher expression levels on CIK cells compared with isotype, expression profiles of CCR4, CCR5 and CCR7 did not show significant changes compared with isotype. (B) Dynamic changes of chemokine receptors CCR4, CCR5, CCR7, CXCR3 and CXCR4 of CIK cells on D7, 14, 21 and 28 were detected in patients with CRC and HD CIK cells via flow cytometry. The result revealed that expression levels of CXCR3 and CXCR4 were significantly higher on CIK cells cultured from CRC compared with HD at all the time points analyzed, while the expression levels of CCR4, CCR5 and CCR7 were significantly higher on CIK cells cultured from CRC compared with HD at different time points analyzed (CCR4 on D7 and D28; CCR5 on D28; CCR7 on D7, D21 and D28). All comparisons were performed using unpaired Student''s t-test with Welch''s correction. *P
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Paclitaxel dysregulates CD4 + and CD8 + T-cell subpopulations in an eIF4E-dependent manner. (A) Gating strategy used for flow cytometry of lymphocytes from popliteal and inguinal lymph nodes. T-cells were separated from the whole lymphoid cells population using CD3. CD3 + cells were then gated for CD4 + T-cell and CD8 + T-cells. For each of these subsets, cells were further gated for CCR7 or CD25 and CD44. (B) Quantification of CD4 + T-cells from the CD3 population from male mice. (C) CCR7 + T-cells from isolated CD4 + population in males, ** p 0.0063. (D) CD4 + cells gated for CD25 and/or CD44 in males. (E) Quantification of CD4 + T-cells from the CD3 + population from female mice, * p 0.0101. (F) CCR7 + T-cells from isolated CD4 + population in female mice, * p 0.0250. (G) CD4 + cells gated for CD25 and/or CD44 in female mice, ** p 0.0011. (H) Quantification of CD8 + T-cells from the CD3 + population from male mice. (I) CCR7 + T-cells from isolated CD8 + population in males, * p 0.0433. (J) CD8 + cells gated for CD25 and/or CD44 in males, * p 0.0396. (K) Quantification of CD8 + T-cells from the CD3 population from female mice, * p 0.041. (L) CCR7 + T-cells from isolated CD8 + population in female mice, ** p 0.0097. (M) CD8 + cells gated for CD25 and/or CD44 in female mice. All data are presented as mean +- standard error of the mean, n = 3 mice each for male/female WT vehicle-treated and WT paclitaxel groups, n = 4 mice each for male/female eIF4E S209A vehicle-trea
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Effect of BYBG on CD4+ T cells. CD4+ T cell concentrations were determined using peripheral blood mononuclear cells (PBMC) isolated from human whole blood samples. PBMCs were analyzed using flow cytometry to determine total ( A , CD3+/CD4+), T EM ( B , CCR7-/CD45RA-), and T CM ( C , CCR7+/CD45RA-) concentrations. Placebo (black bars) and BYBG (green bars) are represented separately and were statistically compared. Values represent the mean +- SEM. * indicates BYBG significantly different than placebo ( p < 0.05).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 Effect of BYBG on CD8+ T cells. CD8+ T cell concentrations were determined using peripheral blood mononuclear cells (PBMC) isolated from human whole blood samples. PBMCs were analyzed using flow cytometry to determine total ( A , CD3+/CD8+), T EM ( B , CCR7-/CD45RA-), and T CM ( C , CCR7+/CD45RA-), and T EMRA ( D , CCR7-/CD45RA+) concentrations. Placebo (black bars) and BYBG (green bars) are represented separately and were statistically compared. Values represent the mean +- SEM. * indicates BYBG significantly different than placebo ( p < 0.05).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 HBV-related samples display higher proportion of effector/memory CD8+ T cells by flow cytometry assay. ( A ) Examples of CD3, CD4 and CCR7 staining to determine effector/memory CD4+ T cells. ( B ) Examples of CD3, CD8 and CCR7 staining to determine effector/memory CD8+ T cells. ( C ) The average proportion of effector/memory CD4+ T cells and CD8+ T cells in HBV and control groups (*p
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 BCA regulate MPhi polarization in vitro and in vivo . (A,B) Raw246.7 cells were treated with 4 mug/ml BCA for 2 h. Then, the cells were infected with Salmonella at MOI = 10:1 for 2 h. (A) The expression of surface markers (CCR7, CD86, CD163, and CD206) was determined by flow cytometry. The results are presented as MFI. (B) TNF-alpha and IL-10 in the supernatants were detected by ELISA. ** P < 0.01, *** P < 0.001. The data are representative of three experiments with similar results. (C,D) Mice were infected by intragastric administration of an overnight culture of Salmonella (10 5 bacteria in 0.1 ml PBS) through a gavage tube and then treated with 6.25 mg/kg BCA intragastrically by gavage daily (5 mice for each group). On the 5th day p.i., the mice were sacrificed and the peritoneal fluid was collected. (C) The expression of surface markers (CCR7, CD86, CD163, and CD206) in mouse peritoneal MPhis were determined by flow cytometry The results are presented as MFI. (D) TNF-alpha and IL-10 in ascitic fluid were detected by ELISA. * P < 0.05, ** P < 0.01, *** P < 0.001.
