Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 47-1979-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD197 (CCR7) Monoclonal Antibody (3D12), APC-eFluor 780, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 3D12 monoclonal antibody reacts with human CCR7, also known as EBI-1 and CD197. CCR7 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains and functions as a receptor for 6Ckine/SLC (secondary lymphoid-tissue chemokine), CCL19 and CCL21. CCR7 has been shown to be internalized via clathrin-coated pits and the majority recycled back to the plasma membrane. CCR7 is expressed on T cells and can be used to distinguish populations of naive from central and effector memory T cells. CCR7 has been shown to play a role in migration of memory T cells to inflamed tissue. Expression of CCR7 is also found on DC's. During DC maturation CCR7 expression increases and is thought to be involved in a variety of functions: chemotaxis to the lymph node, cellular architecture, rate of endocytosis, survival and maturation. Expression of CCR7 on the cell surface can be down regulated upon ligand binding. Applications Reported: This 3D12 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 3D12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the staining incubation time be increased to at least 45 minutes at 2-8°C. APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- 3D12
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Vaccine Induction of Lymph Node-Resident Simian Immunodeficiency Virus Env-Specific T Follicular Helper Cells in Rhesus Macaques.
Temporal Dynamics of CD8+ T Cell Effector Responses during Primary HIV Infection.
PD-1+Tim-3+ CD8+ T Lymphocytes Display Varied Degrees of Functional Exhaustion in Patients with Regionally Metastatic Differentiated Thyroid Cancer.
Select host restriction factors are associated with HIV persistence during antiretroviral therapy.
Comprehensive analysis of dengue virus-specific responses supports an HLA-linked protective role for CD8+ T cells.
Differential localization of T-bet and Eomes in CD8 T cell memory populations.
Role of TLX1 in T-cell acute lymphoblastic leukaemia pathogenesis.
Proliferation and differentiation potential of human CD8+ memory T-cell subsets in response to antigen or homeostatic cytokines.
Two subsets of memory T lymphocytes with distinct homing potentials and effector functions.
Vargas-Inchaustegui DA, Demers A, Shaw JM, Kang G, Ball D, Tuero I, Musich T, Mohanram V, Demberg T, Karpova TS, Li Q, Robert-Guroff M
Journal of immunology (Baltimore, Md. : 1950) 2016 Feb 15;196(4):1700-10
Journal of immunology (Baltimore, Md. : 1950) 2016 Feb 15;196(4):1700-10
Temporal Dynamics of CD8+ T Cell Effector Responses during Primary HIV Infection.
Demers KR, Makedonas G, Buggert M, Eller MA, Ratcliffe SJ, Goonetilleke N, Li CK, Eller LA, Rono K, Maganga L, Nitayaphan S, Kibuuka H, Routy JP, Slifka MK, Haynes BF, McMichael AJ, Bernard NF, Robb ML, Betts MR
PLoS pathogens 2016 Aug;12(8):e1005805
PLoS pathogens 2016 Aug;12(8):e1005805
PD-1+Tim-3+ CD8+ T Lymphocytes Display Varied Degrees of Functional Exhaustion in Patients with Regionally Metastatic Differentiated Thyroid Cancer.
Severson JJ, Serracino HS, Mateescu V, Raeburn CD, McIntyre RC Jr, Sams SB, Haugen BR, French JD
Cancer immunology research 2015 Jun;3(6):620-30
Cancer immunology research 2015 Jun;3(6):620-30
Select host restriction factors are associated with HIV persistence during antiretroviral therapy.
Abdel-Mohsen M, Wang C, Strain MC, Lada SM, Deng X, Cockerham LR, Pilcher CD, Hecht FM, Liegler T, Richman DD, Deeks SG, Pillai SK
AIDS (London, England) 2015 Feb 20;29(4):411-20
AIDS (London, England) 2015 Feb 20;29(4):411-20
Comprehensive analysis of dengue virus-specific responses supports an HLA-linked protective role for CD8+ T cells.
Weiskopf D, Angelo MA, de Azeredo EL, Sidney J, Greenbaum JA, Fernando AN, Broadwater A, Kolla RV, De Silva AD, de Silva AM, Mattia KA, Doranz BJ, Grey HM, Shresta S, Peters B, Sette A
Proceedings of the National Academy of Sciences of the United States of America 2013 May 28;110(22):E2046-53
Proceedings of the National Academy of Sciences of the United States of America 2013 May 28;110(22):E2046-53
Differential localization of T-bet and Eomes in CD8 T cell memory populations.
McLane LM, Banerjee PP, Cosma GL, Makedonas G, Wherry EJ, Orange JS, Betts MR
Journal of immunology (Baltimore, Md. : 1950) 2013 Apr 1;190(7):3207-15
Journal of immunology (Baltimore, Md. : 1950) 2013 Apr 1;190(7):3207-15
Role of TLX1 in T-cell acute lymphoblastic leukaemia pathogenesis.
Riz I, Hawley TS, Johnston H, Hawley RG
British journal of haematology 2009 Apr;145(1):140-3
British journal of haematology 2009 Apr;145(1):140-3
Proliferation and differentiation potential of human CD8+ memory T-cell subsets in response to antigen or homeostatic cytokines.
Geginat J, Lanzavecchia A, Sallusto F
Blood 2003 Jun 1;101(11):4260-6
Blood 2003 Jun 1;101(11):4260-6
Two subsets of memory T lymphocytes with distinct homing potentials and effector functions.
Sallusto F, Lenig D, Förster R, Lipp M, Lanzavecchia A
Nature 1999 Oct 14;401(6754):708-12
Nature 1999 Oct 14;401(6754):708-12
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD45RA FITC (Product # 11-0458-42) and Rat IgG2a K Isotype Control APC-eFluor® 780 (Product # 47-4321-82) (left) or Anti-Human CD197 (CCR7) APC-eFluor® 780 (right). Cells in the lymphocyte gate were used for analysis.