HPA070314

antibody from Atlas Antibodies

Targeting: JAK3 JAK-3, JAK3_HUMAN, JAKL, L-JAK, LJAK

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA070314

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA070314, RRID:AB_2686251

Product name

Anti-JAK3

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

ETFHVGLPGALGGHDGLGLLRVAGDGGIAWTQGEQ
EVLQPFCDFPEIVDISIKQAPRVGPAGEHRLVTVT
RTDNQILEAEFPGL


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line REH shows localization to cytosol.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human appendix and liver tissues using Anti-JAK3 antibody. Corresponding JAK3 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in a fraction of cells in red pulp.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human appendix shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver shows low expression as expected.

Sample type

HUMAN