Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [3]
- Flow cytometry [1]
- Other assay [2]
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Validation data
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- Product number
- MA5-15561 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- JAK3 Monoclonal Antibody (5H2)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15561 targets JAK3 in indirect ELISA, FACS, IF, and WB applications and shows reactivity with Human and mouse samples. The MA5-15561 immunogen is purified recombinant fragment of human JAK3 expressed in E. Coli. MA5-15561 detects JAK3 which has a predicted molecular weight of approximately 125kDa.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5H2
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Selective Inhibition of Janus Kinase 3 Has No Impact on Infarct Size or Neurobehavioral Outcomes in Permanent Ischemic Stroke in Mice.
DeMars KM, Pacheco SC, Yang C, Siwarski DM, Candelario-Jalil E
Frontiers in neurology 2017;8:363
Frontiers in neurology 2017;8:363
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of 3T3-L1 cells using JAK3 monoclonal antibody (Product # MA5-15561) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with DY-554 phalloidin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa (left) and HepG2 (right) cells using JAK3 monoclonal antibody (Product # MA5-15561) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with DY-554 phalloidin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of 3T3-L1 cells using JAK3 monoclonal antibody (Product # MA5-15561) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with DY-554 phalloidin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using JAK3 monoclonal antibody (Product # MA5-15561) (blue) and negative control (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL