100-401-152

antibody from Rockland Immunochemicals, Inc.

Targeting: CCNB1 CCNB

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

100-401-152

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#100-401-152, RRID:AB_2072270

Product name

Anti-Cyclin B1 (RABBIT) Antibody - 100-401-152

Antibody type

Polyclonal

Vial size

100 µl

References

Submitted references

Epicardial-derived adrenomedullin drives cardiac hyperplasia during embryogenesis.

Wetzel-Strong SE, Li M, Klein KR, Nishikimi T, Caron KM
Developmental dynamics : an official publication of the American Association of Anatomists 2014 Feb;243(2):243-56

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot analysis using Rockland's Anti-Cyclin B₁ antibody shows detection of Cyclin B₁ present in asynchronous HeLa cell lysates.  Comparison to a molecular weight marker indicates a band of ~55 kDa corresponding to human Cyclin B₁ (arrowhead).  Approximately 50 µg of lysate was loaded on to a 7% SDS-PAGE gel for separation. After transfer to nitrocellulose, the blot was incubated with a 1:500 dilution of the antibody for 1 h at room temperature.  Detection occurred using a 1:10,000 of HRP conjugated Goat-a-Rabbit IgG (p/n 611-103-122).  Personal communication, Luca D'Agostino, Temple University, Philadelphia, PA.

Validation comment

Western Blot