600-401-464

antibody from Rockland Immunochemicals, Inc.

Targeting: CCNB1 CCNB

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-464

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-464, RRID:AB_2072269

Product name

Anti-Cyclin B1 pS126 (RABBIT) Antibody - 600-401-464

Antibody type

Polyclonal

Vial size

100 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot using Rockland's Affinity Purified anti-Cyclin B₁ pS126 antibody shows detection of a band ~48 kDa corresponding to phosphorylated human Cyclin B₁ (arrowheads) in various whole cell lysates.  Lysates tested were lane 1 - Hela (cervical carcinoma), lane 2 - H23 (lung carcinoma), lane 3 - Hep3b (Hepatocarcinoma), lane 4 - T98G (Glioblastoma) and lane 5 - Daudi (B cell lymphoblast). Each lane contains approximately 50 µg of lysates, separated by 12% SDS-PAGE using a 5% stack run at 100 volts until the dye front cleared the bottom of the gel.  Transfer occurred overnight at 4° C at 15 mAmps.  The membrane was blocked with 5% non-fat dry milk in TTBS for 1 h at room temperature followed by addition of a 1:100 dilution of the antibody allowed to react for 2h at room temperature.  After washes with TTBS a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG [H&L] MX (611-103-122) was added for 1 h at room temperature.  After additional washes the membrane was incubated with ECL mix 1:1 for ~3 min.  Excess detection solution was drained off and the membrane was exposed to Kodak film X-omat blue XB-1 for about 20 sec. Other detection systems will yield similar results.  Personnel Communication, Luca Cote, Temple U.

Validation comment

Western Blot