Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF4809 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal IkB-alpha Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human IkB-alpha when phosphorylated at S32/36.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Cordycepin Enhances Radiosensitivity in Oral Squamous Carcinoma Cells by Inducing Autophagy and Apoptosis Through Cell Cycle Arrest.
An ultrasensitive fiveplex activity assay for cellular kinases.
Aggregation of Human Trophoblast Cells into Three-Dimensional Culture System Enhances Anti-Inflammatory Characteristics through Cytoskeleton Regulation.
Open reading frame 3 of genotype 1 hepatitis E virus inhibits nuclear factor-κappa B signaling induced by tumor necrosis factor-α in human A549 lung epithelial cells.
Ho SY, Wu WS, Lin LC, Wu YH, Chiu HW, Yeh YL, Huang BM, Wang YJ
International journal of molecular sciences 2019 Oct 28;20(21)
International journal of molecular sciences 2019 Oct 28;20(21)
An ultrasensitive fiveplex activity assay for cellular kinases.
Smolko CM, Janes KA
Scientific reports 2019 Dec 19;9(1):19409
Scientific reports 2019 Dec 19;9(1):19409
Aggregation of Human Trophoblast Cells into Three-Dimensional Culture System Enhances Anti-Inflammatory Characteristics through Cytoskeleton Regulation.
Seno K, Munakata Y, Sano M, Kawahara-Miki R, Takahashi H, Ohkuchi A, Iwata H, Kuwayama T, Shirasuna K
International journal of molecular sciences 2018 Aug 8;19(8)
International journal of molecular sciences 2018 Aug 8;19(8)
Open reading frame 3 of genotype 1 hepatitis E virus inhibits nuclear factor-κappa B signaling induced by tumor necrosis factor-α in human A549 lung epithelial cells.
Xu J, Wu F, Tian D, Wang J, Zheng Z, Xia N
PloS one 2014;9(6):e100787
PloS one 2014;9(6):e100787
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Phospho-IkB-alpha (S32/S36) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 20 ng/mL Recombinant Human TNF-alpha (Catalog # 210-TA) for 5 minutes. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human Phospho-IkB-alpha (S32/S36) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4809) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-IkB-alpha (S32/S36) was detected at approximately 44 kDa (as indicated). For additional reference, duplicate lysates were probed with 0.1 µg/mL Human IkB-alpha Monoclonal Antibody (lower panel, Catalog # MAB4299). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.