Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- GTX110521 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX110521, RRID:AB_1950996
- Product name
- IKB alpha antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references A high-throughput pipeline for validation of antibodies.
Protective Effect of Eupatilin Pretreatment Against Hepatic Ischemia-Reperfusion Injury in Mice.
Substrate Stiffness Regulates Proinflammatory Mediator Production through TLR4 Activity in Macrophages.
Inhibitory effect of litchi (Litchi chinensis Sonn.) flower on lipopolysaccharide-induced expression of proinflammatory mediators in RAW264.7 cells through NF-κB, ERK, and JAK2/STAT3 inactivation.
Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912
Nature methods 2018 Nov;15(11):909-912
Protective Effect of Eupatilin Pretreatment Against Hepatic Ischemia-Reperfusion Injury in Mice.
Lee HM, Jang HJ, Kim SS, Kim HJ, Lee SY, Oh MY, Kwan HC, Jang DS, Eom DW
Transplantation proceedings 2016 May;48(4):1226-33
Transplantation proceedings 2016 May;48(4):1226-33
Substrate Stiffness Regulates Proinflammatory Mediator Production through TLR4 Activity in Macrophages.
Previtera ML, Sengupta A
PloS one 2015;10(12):e0145813
PloS one 2015;10(12):e0145813
Inhibitory effect of litchi (Litchi chinensis Sonn.) flower on lipopolysaccharide-induced expression of proinflammatory mediators in RAW264.7 cells through NF-κB, ERK, and JAK2/STAT3 inactivation.
Yang DJ, Chang YY, Lin HW, Chen YC, Hsu SH, Lin JT
Journal of agricultural and food chemistry 2014 Apr 16;62(15):3458-65
Journal of agricultural and food chemistry 2014 Apr 16;62(15):3458-65
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HT-29 whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with IKB alpha antibody (GTX110521) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IKB alpha antibody detects IKB alpha protein by western blot analysis.A. 30 ?g BCL-1 whole cell lysate/extract B. 30 ?g C2C12 whole cell lysate/extrac10% SDS-PAGEIKB alpha antibody (GTX110521) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: HepG2 (GTX27900) 10% SDS PAGE GTX110521 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Untreated (¡V) and treated (+) NIH-3T3 whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with IKB alpha antibody (GTX110521) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with IKB alpha antibody (GTX110521) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HT-29 whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with IKB alpha antibody (GTX110521) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using IkappaB-alpha(GTX110521) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IKB alpha antibody detects NFKBIA protein at nucleus on U251 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U251 xenograft. IKB alpha antibody (GTX110521) dilution: 1:500.