- GTX110521 - GeneTex NFKBIA antibody

Submitted references A high-throughput pipeline for validation of antibodies.

Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
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Enhanced validation

Submitted by: GeneTex (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Non-transfected (¡V) and transfected (+) HT-29 whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with IKB alpha antibody (GTX110521) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
KD Reagent Type: siRNA
Downregulation: >50%
KD Reagent Prod page: KD Reagent Prod page
KD Reagent Provider: shRNA reagents was provided courtesy of cooperative labs.
KD Reagent Prod no: Proprietary.
Antibody Lot Number: 41983

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: IKB alpha antibody detects IKB alpha protein by western blot analysis.A. 30 ?g BCL-1 whole cell lysate/extract B. 30 ?g C2C12 whole cell lysate/extrac10% SDS-PAGEIKB alpha antibody (GTX110521) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ?g of whole cell lysate) A: HepG2 (GTX27900) 10% SDS PAGE GTX110521 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Untreated (¡V) and treated (+) NIH-3T3 whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with IKB alpha antibody (GTX110521) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with IKB alpha antibody (GTX110521) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) HT-29 whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with IKB alpha antibody (GTX110521) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using IkappaB-alpha(GTX110521) antibody at 1:200 dilution.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: IKB alpha antibody detects NFKBIA protein at nucleus on U251 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U251 xenograft. IKB alpha antibody (GTX110521) dilution: 1:500.

GTX110521

Antibody data