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- Antibody Data
- Antigen structure
- References [1]
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- Western blot [1]
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- Product number
- NB100-56065 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56065, RRID:AB_839166
- Product name
- Mouse Monoclonal IkB-alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. IkBa (39A1413 ) [Ser32/Ser36]
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Porcine
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Mild hypothermia inhibits nuclear factor-kappaB translocation in experimental stroke.
Han HS, Karabiyikoglu M, Kelly S, Sobel RA, Yenari MA
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2003 May;23(5):589-98
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2003 May;23(5):589-98
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) [Biotin] [NB100-56065] - Jurkat cells were treated for 30 min with 100 ug/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal) a Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation) followed by incubation with (lanes 2 & 4) or without 1 nM TNF-a (1 & 3). The membranes were blotted with NB100-56065(lanes 1 &2) or NB100-56507 (that recognizes both non-phospho and phosphorylated forms of IkBa) and immunoreactivity was detected by ECL. The data shows that NB100-56065 detects specifically the phosphorylated form of IkBa.
