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Learn10268-1-AP
antibody from Invitrogen Antibodies
Targeting: NFKBIA
IkappaBalpha, IKBA, MAD-3, NFKBI
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [6]
- Immunocytochemistry [2]
- Immunohistochemistry [8]
- Flow cytometry [3]
- Other assay [1]
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- Product number
- 10268-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IkB alpha Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.16 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 10268-1-AP ( IkB alpha antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10268-1-AP (IkB alpha antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A431 cells were subjected to SDS PAGE followed by western blot with 10268-1-AP (IkB alpha antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 10268-1-AP (IkB alpha antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- Pig liver tissue were subjected to SDS PAGE followed by western blot with 10268-1-AP (IkB alpha antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- Mouse liver tissue were subjected to SDS PAGE followed by western blot with 10268-1-AP (IkB alpha antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 10268-1-AP (IkB alpha antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 10268-1-AP (IkB alpha antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 10268-1-AP (IkB alpha antibody at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 10268-1-AP (IkB alpha antibody at dilution of 1:200 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 10268-1-AP (IkB alpha antibody at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 10268-1-AP (IkB alpha antibody at dilution of 1:200 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human stomach tissue slide using 10268-1-AP (IkB alpha antibody at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human stomach tissue slide using 10268-1-AP (IkB alpha antibody at dilution of 1:200 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 10268-1-AP ( IkB alpha antibody at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 10268-1-AP ( IkB alpha antibody at dilution of 1:200 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were stained with 0.2ug IkB alpha antibody (10268-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 NIH/3T3 cells were stained with 0.2 ug IkB alpha antibody (10268-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) with dilution 1:1500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were intracellularly stained with 0.4 µg Anti-Human IkB Alpha (Product # 10268-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (red), or 0.4 µg Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-IkB alpha (IP:10268-1-AP, 4ug; Detection:10268-1-AP 1:1000) with HeLa cells lysate 4000ug.
