Antibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Western blot [2]
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- Product number
- NB100-56724 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56724, RRID:AB_839165
- Product name
- Mouse Monoclonal IkB-alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. IkBa (39A1413 ) [Ser32/Ser36]
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Porcine
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Immunomodulatory effect of low molecular-weight seleno-aminopolysaccharide on immunosuppressive mice.
uPA-derived peptide, Å6 is involved in the suppression of lipopolysaccaride-promoted inflammatory osteoclastogenesis and the resultant bone loss.
Immunomodulatory effect of low molecular-weight seleno-aminopolysaccharides in intestinal epithelial cells.
Role of ferulic acid in the amelioration of ionizing radiation induced inflammation: a murine model.
Hypoxia-inducible factors are required for chemotherapy resistance of breast cancer stem cells.
Botanical Extracts from Rosehip (Rosa canina), Willow Bark (Salix alba), and Nettle Leaf (Urtica dioica) Suppress IL-1β-Induced NF-κB Activation in Canine Articular Chondrocytes.
Cardioprotection afforded by inducible nitric oxide synthase gene therapy is mediated by cyclooxygenase-2 via a nuclear factor-kappaB dependent pathway.
B cell maturation antigen, the receptor for a proliferation-inducing ligand and B cell-activating factor of the TNF family, induces antigen presentation in B cells.
B cell maturation antigen, the receptor for a proliferation-inducing ligand and B cell-activating factor of the TNF family, induces antigen presentation in B cells.
Rituximab (chimeric anti-CD20 monoclonal antibody) inhibits the constitutive nuclear factor-{kappa}B signaling pathway in non-Hodgkin's lymphoma B-cell lines: role in sensitization to chemotherapeutic drug-induced apoptosis.
Highly purified lipoteichoic acid activates neutrophil granulocytes and delays their spontaneous apoptosis via CD14 and TLR2.
Mild hypothermia inhibits nuclear factor-kappaB translocation in experimental stroke.
PC-SPES: a potent inhibitor of nuclear factor-kappa B rescues mice from lipopolysaccharide-induced septic shock.
Wen ZS, Tang Z, Gu LX, Xiang XW, Qu YL
International journal of biological macromolecules 2019 Feb 15;123:1278-1288
International journal of biological macromolecules 2019 Feb 15;123:1278-1288
uPA-derived peptide, Å6 is involved in the suppression of lipopolysaccaride-promoted inflammatory osteoclastogenesis and the resultant bone loss.
Kanno Y, Maruyama C, Matsuda A, Ishisaki A
Immunity, inflammation and disease 2017 Sep;5(3):289-299
Immunity, inflammation and disease 2017 Sep;5(3):289-299
Immunomodulatory effect of low molecular-weight seleno-aminopolysaccharides in intestinal epithelial cells.
Gu LX, Wen ZS, Xiang XW, Ma L, Wang XB, Ma JY, Qu YL
International journal of biological macromolecules 2017 Jun;99:570-577
International journal of biological macromolecules 2017 Jun;99:570-577
Role of ferulic acid in the amelioration of ionizing radiation induced inflammation: a murine model.
Das U, Manna K, Sinha M, Datta S, Das DK, Chakraborty A, Ghosh M, Saha KD, Dey S
PloS one 2014;9(5):e97599
PloS one 2014;9(5):e97599
Hypoxia-inducible factors are required for chemotherapy resistance of breast cancer stem cells.
Samanta D, Gilkes DM, Chaturvedi P, Xiang L, Semenza GL
Proceedings of the National Academy of Sciences of the United States of America 2014 Dec 16;111(50):E5429-38
Proceedings of the National Academy of Sciences of the United States of America 2014 Dec 16;111(50):E5429-38
Botanical Extracts from Rosehip (Rosa canina), Willow Bark (Salix alba), and Nettle Leaf (Urtica dioica) Suppress IL-1β-Induced NF-κB Activation in Canine Articular Chondrocytes.
Shakibaei M, Allaway D, Nebrich S, Mobasheri A
Evidence-based complementary and alternative medicine : eCAM 2012;2012:509383
Evidence-based complementary and alternative medicine : eCAM 2012;2012:509383
Cardioprotection afforded by inducible nitric oxide synthase gene therapy is mediated by cyclooxygenase-2 via a nuclear factor-kappaB dependent pathway.
Li Q, Guo Y, Tan W, Ou Q, Wu WJ, Sturza D, Dawn B, Hunt G, Cui C, Bolli R
Circulation 2007 Oct 2;116(14):1577-84
Circulation 2007 Oct 2;116(14):1577-84
B cell maturation antigen, the receptor for a proliferation-inducing ligand and B cell-activating factor of the TNF family, induces antigen presentation in B cells.
Yang M, Hase H, Legarda-Addison D, Varughese L, Seed B, Ting AT
Journal of immunology (Baltimore, Md. : 1950) 2005 Sep 1;175(5):2814-24
Journal of immunology (Baltimore, Md. : 1950) 2005 Sep 1;175(5):2814-24
B cell maturation antigen, the receptor for a proliferation-inducing ligand and B cell-activating factor of the TNF family, induces antigen presentation in B cells.
Yang M, Hase H, Legarda-Addison D, Varughese L, Seed B, Ting AT
Journal of immunology (Baltimore, Md. : 1950) 2005 Sep 1;175(5):2814-24
Journal of immunology (Baltimore, Md. : 1950) 2005 Sep 1;175(5):2814-24
Rituximab (chimeric anti-CD20 monoclonal antibody) inhibits the constitutive nuclear factor-{kappa}B signaling pathway in non-Hodgkin's lymphoma B-cell lines: role in sensitization to chemotherapeutic drug-induced apoptosis.
Jazirehi AR, Huerta-Yepez S, Cheng G, Bonavida B
Cancer research 2005 Jan 1;65(1):264-76
Cancer research 2005 Jan 1;65(1):264-76
Highly purified lipoteichoic acid activates neutrophil granulocytes and delays their spontaneous apoptosis via CD14 and TLR2.
Lotz S, Aga E, Wilde I, van Zandbergen G, Hartung T, Solbach W, Laskay T
Journal of leukocyte biology 2004 Mar;75(3):467-77
Journal of leukocyte biology 2004 Mar;75(3):467-77
Mild hypothermia inhibits nuclear factor-kappaB translocation in experimental stroke.
Han HS, Karabiyikoglu M, Kelly S, Sobel RA, Yenari MA
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2003 May;23(5):589-98
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2003 May;23(5):589-98
PC-SPES: a potent inhibitor of nuclear factor-kappa B rescues mice from lipopolysaccharide-induced septic shock.
Ikezoe T, Yang Y, Heber D, Taguchi H, Koeffler HP
Molecular pharmacology 2003 Dec;64(6):1521-9
Molecular pharmacology 2003 Dec;64(6):1521-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) [NB100-56724] - Jurkat cells were treated for 30 min with 100 ug/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal) - a Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation) followed by incubation with (lane 2) or without 1 nM TNF-a (lane 1). The membrane was blotted with NB100-56724 and immunoreactivity was detected by ECL. The data shows that NB100-56724 detects specifically the phosphorylated form of IkBa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) [NB100-56724] - Analysis using the Biotin conjugate of NB100-56724. Detection of Jurkat cells were treated for 30 min with 100 ug/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal; a Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation) followed by incubation with (lanes 2 & 4) or without 1 nM TNF-a (1 & 3). The membranes were blotted with this antibody.