Ab109691

antibody from Aladdin Scientific

Targeting: NFKBIA IkappaBalpha, IKBA, MAD-3, NFKBI

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

Ab109691

Provider

Aladdin Scientific

Product name

Recombinant IKB alpha Antibody

Antibody type

Recombinant

Description

Rabbit anti Human IKB alpha Antibody, Recombinant, could be used for WB and so on.Application:WB: 1/500-1/1000Protein FunctionInhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.

Reactivity

Human, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

AA 308-317


Vial size

100_l,10_l,1ml,50_l

Concentration

2 mg/ml

Storage

Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Western blot

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Recombinant IKB alpha Antibody (Ab109691) - Western Blot All lanes: Recombinant IKB alpha Antibody (Ab109691) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 61.9 s