GTX25682

antibody from GeneTex

Targeting: NFKBIA IkappaBalpha, IKBA, MAD-3, NFKBI

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX25682

Provider

GeneTex

Proper citation

GeneTex Cat#GTX25682, RRID:AB_380650

Product name

IKB alpha (phospho Ser32/Ser36) antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

References

Submitted references

Protective Effect of Eupatilin Pretreatment Against Hepatic Ischemia-Reperfusion Injury in Mice.

Lee HM, Jang HJ, Kim SS, Kim HJ, Lee SY, Oh MY, Kwan HC, Jang DS, Eom DW
Transplantation proceedings 2016 May;48(4):1226-33

---

RRM2B-Mediated Regulation of Mitochondrial Activity and Inflammation under Oxidative Stress.

Cho EC, Kuo ML, Cheng JH, Cheng YC, Hsieh YC, Liu YR, Hsieh RH, Yen Y
Mediators of inflammation 2015;2015:287345

---

Substrate Stiffness Regulates Proinflammatory Mediator Production through TLR4 Activity in Macrophages.

Previtera ML, Sengupta A
PloS one 2015;10(12):e0145813

---

Antiosteoclastogenic activity of silicate-based materials antagonizing receptor activator for nuclear factor kappaB ligand-induced osteoclast differentiation of murine marcophages.

Hung CJ, Kao CT, Chen YJ, Shie MY, Huang TH
Journal of endodontics 2013 Dec;39(12):1557-61

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Peptide Competition and Induction: Extracts prepared from ALLN pretreated Jurkat cells left unstimulated (1, 2) or stimulated with TNF alpha?(3 7) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature and incubated with anti-IKBa pan (1, 3) or?GTX25682?antibody (2, 4-7) overnight at 4?C in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1-4), the non phosphopeptide corresponding to the immunogen (5), a generic phosphoserine-containing peptide (6), or, the phosphopeptide immunogen (7). After washing, membranes were incubated with goat F(ab?)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal method. The data show that the phosphorylation of IKBa is induced by the addition of TNF alpha?and that the peptide corresponding to IKBa [pSpS32/36] blocks the antibody signal, thereby demonstrating the specificity of the antibody. The data also show that treatment with TNF alpha?leads to degradation of IKBa protein (compare lanes 1 and 3), as expected, even in the presence of ALLN.