Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF4809 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Phospho-IkB-alpha (S32/S36) Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human IkB-alpha when phosphorylated at S32/36.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Aggregation of Human Trophoblast Cells into Three-Dimensional Culture System Enhances Anti-Inflammatory Characteristics through Cytoskeleton Regulation.
Open reading frame 3 of genotype 1 hepatitis E virus inhibits nuclear factor-κappa B signaling induced by tumor necrosis factor-α in human A549 lung epithelial cells.
Seno K, Munakata Y, Sano M, Kawahara-Miki R, Takahashi H, Ohkuchi A, Iwata H, Kuwayama T, Shirasuna K
International journal of molecular sciences 2018 Aug 8;19(8)
International journal of molecular sciences 2018 Aug 8;19(8)
Open reading frame 3 of genotype 1 hepatitis E virus inhibits nuclear factor-κappa B signaling induced by tumor necrosis factor-α in human A549 lung epithelial cells.
Xu J, Wu F, Tian D, Wang J, Zheng Z, Xia N
PloS one 2014;9(6):e100787
PloS one 2014;9(6):e100787
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of Human Phospho-IkB-alpha (S32/S36) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 20 ng/mL Recombinant Human TNF-alpha (Catalog # 210-TA) for 5 minutes. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human Phospho-IkB-alpha (S32/S36) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4809) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-IkB-alpha (S32/S36) was detected at approximately 44 kDa (as indicated). For additional reference, duplicate lysates were probed with 0.1 µg/mL Human IkB-alpha Monoclonal Antibody (lower panel, Catalog # MAB4299). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.