AP7961a

antibody from Abgent

Targeting: MAP2K2 MEK2, PRKMK2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP7961a

Provider

Abgent

Proper citation

Abgent Cat#AP7961a, RRID:AB_353871

Product name

MEK2 (MAP2K2) Antibody (N-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"MEK2 (MAP2K2) Antibody (N-term) (Cat. #AP7961a) western blot analysis in Hela,MCF-7,rat C6 cell line and mouse Liver,rat Brain tissue lysates (35ug/lane).This demonstrates the MEK2 (MAP2K2) antibody detected the MEK2 (MAP2K2) protein (arrow)."

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Fluorescent confocal image of U251 cell stained with MEK2 (MAP2K2) Antibody (N-term)(Cat#AP7961a).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MEK2 (MAP2K2) primary antibody (1:25, 1 h at 37!). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37!).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37!). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).MEK2 (MAP2K2) immunoreactivity is localized to Cytoplasm significantly."

Primary Ab dilution

1:10~50