GTX111338

antibody from GeneTex

Targeting: MAP2K2 MEK2, PRKMK2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX111338

Provider

GeneTex

Proper citation

GeneTex Cat#GTX111338, RRID:AB_10722030

Product name

MEK2 antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Storage

Keep as concentrated solution. Aliquot and store at -20?C or below. Avoid multiple freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE GTX111338 diluted at 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: NIH-3T3 B: BCL-1 10% SDS PAGE GTX111338 diluted at 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: PC-12 10% SDS PAGE GTX111338 diluted at 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with MEK2 antibody (GTX111338) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with MEK2 antibody (GTX111338) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescence analysis of methanol-fixed HCT116, using MEK2(GTX111338) antibody at 1:500 dilution.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using MEK2(GTX111338) antibody at 1:500 dilution.