GTX24757

antibody from GeneTex

Targeting: MAP2K2 MEK2, PRKMK2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX24757

Provider

GeneTex

Proper citation

GeneTex Cat#GTX24757, RRID:AB_380408

Product name

MEK2 (phospho Thr394) antibody

Antibody type

Polyclonal

Reactivity

Mouse, Rat

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Extracts of A431 cells treated with with 200 ng/mL EGF for 15 minutes were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membranes were left untreated (1-4, 6) or treated with lambda phosphatase (5), blocked with a 4% BSA-TBST buffer for one hour at room temperature, and incubated with the MEK2 [pT394] (human) antibody for two hours at room temperature in a 1% BSA-TBST, following prior incubation with: no peptide (1, 5, 6), the non-phosphopeptide corresponding to the phosphoepptide immunogen (2), a generic phosphothreonine-containing peptide (3) or the phosphopeptide immunogen (4). After washing, the membranes were incubated with goat F(ab)2 anti-rabbit IgG: HRP conjμgate and signals were detected. The data show that only the phosphopeptide corresponding to MEK2 [pT394] (human) blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, further verifying that the antibody is phospho-specific.