TA321908

antibody from OriGene

Targeting: MAP2K2 MEK2, PRKMK2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA321908

Provider

OriGene

Product name

Anti-MAP2K2 Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to a region derived from 20-34 amino acids of Human mitogen-activated protein kinase kinase 2

Description

Anti-MAP2K2 Rabbit Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µl

Concentration

0.8mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted band size: 44 kDa . Positive control: 293T and NIH/3T3 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: 293T cell lysate Lane2: NIH/3T3 cell lysate Lysates: 40 ug per lane Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds)

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted cell location: Cytoplasm. Positive control: Human lung cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using MAP2K2 antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification:x200)

Validation comment

IHC