TA505716

antibody from OriGene

Targeting: MAP2K2 MEK2, PRKMK2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA505716

Provider

OriGene

Proper citation

OriGene Cat#TA505716, RRID:AB_2623315

Product name

MAP2K2 (MEK2 ) mouse monoclonal antibody, clone OTI5G8 (formerly 5G8)

Antibody type

Monoclonal

Description

MAP2K2 (MEK2 ) mouse monoclonal antibody, clone OTI5G8 (formerly 5G8)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

MAP2K2

Isotype

IgG

Antibody clone number

OTI5G8

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K2 (RC201827, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K2.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-MAP2K2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-MAP2K2 mouse monoclonal antibody (TA505716) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAP2K2(RC201827).

Validation comment

IF