LS-C745392

antibody from LSBio

Targeting: MAP2K2 MEK2, PRKMK2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C745392

Provider

LSBio

Product name

MAP2K2 / MKK2 / MEK2 Antibody (C-Terminus) LS-C745392

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Storage

Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western Blot of Anti-MEK2 C-term Antibody. Lane 1: MEK1 rec lysate. Lane 2: MEK2 rec lysate. Load: 10ug. Primary Antibody: Anti-MEK2 at 1µg/mL overnight at 4°C. Secondary Antibody: Goat Anti-Rabbit Peroxidase Conjugated Antibody at 1:40,000 for 30 min at RT. Blocking: BlockOut Universal Blocking blocking buffer MB-073. Predicted MW: 45kDa.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry with anti-MEK2 (N-Term) antibody showing positive staining in human glioblastoma tissue at 20x and 40x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval and then incubated with rabbit anti-MEK2 (N-Term) antibody at 1:100 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3?-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.