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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MA5-15135 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MEK1/MEK2 Monoclonal Antibody (J.653.9)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- J.653.9
- Vial size
- 100 µL
- Concentration
- 2 µg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MEK1/2 in extracts from NIH/3T3 and PC12 cells using MEK1/2 monoclonal antibody (Product # MA5-15135).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MEK1/2 in untreated HeLa cells using a MEK1/2 monoclonal antibody (Product # MA5-15135) (red). Actin filaments have been labeled with FITC-conjugated phalloidin. DNA is labeled using a fluorescent blue dye.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of MEK1/2 in paraffin-embedded human colon carcinoma using a MEK1/2 monoclonal antibody (Product # MA5-15135).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MEK1/2 in U0126-treated (blue) or PMA-treated (green) Jurkat cells using a MEK1/2 monoclonal antibody (Product # MA5-15135) compared to a nonspecific negative control antibody (red).
