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LearnAM32548PU-N
antibody from Acris Antibodies GmbH
Targeting: PTK2B
CADTK, CAKB, FAK2, PTK, PYK2, RAFTK
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoprecipitation [1]
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- Product number
- AM32548PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti FAK2 / PTK2B pTyr402
- Antibody type
- Monoclonal
- Antigen
- KLH-conjugated, synthetic peptide containing ... DI[pY]AE.. in which pY corresponds to phosphotyrosine 402 of Human Pyk2 (Protein Tyrosine kinase 2).
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- RR102
- Vial size
- 0.2 mg
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Supportive validation
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- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunoprecipitation/Immunoblot Analysis: Pyk2 was immunoprecipitated from untreated (lane 2); EGF (lane 3), bradykinin (lane 4), or KCL (lane 5) treated-PC12 cell lysate using an anti-Pyk2 rabbit polyclonal antiserum or control serum (lane 1). The immunoprecipitated material was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-PYK2 and ananti-rabbit HRP conjugate (top panel) or anti-phospho-Pyk2 (Tyr402) clone RR102 (1μg/ml) and a goat anti-mouse secondary antibody conjugated to HRP (bottom panel). Proteins were visualized using a chemiluminescence detection system. Arrow indicates phospho-Pyk2 (116kDa). Data courtesy of Dr. Hava Avraham.
