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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [8]
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- 44-620G - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-PYK2 (Tyr881) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C
Submitted references The role of proline-rich protein tyrosine kinase 2 in differentiation-dependent signaling in human epidermal keratinocytes.
Role of PYK2 in the development of obesity and insulin resistance.
Heat shock protein 60 inhibits Th1-mediated hepatitis model via innate regulation of Th1/Th2 transcription factors and cytokines.
Heat shock protein 60 inhibits Th1-mediated hepatitis model via innate regulation of Th1/Th2 transcription factors and cytokines.
Implication of phospholipase D2 in oxidant-induced phosphoinositide 3-kinase signaling via Pyk2 activation in PC12 cells.
Glucose activates protein kinase C-zeta /lambda through proline-rich tyrosine kinase-2, extracellular signal-regulated kinase, and phospholipase D: a novel mechanism for activating glucose transporter translocation.
Salicylate Inhibits Phosphorylation of the Nonreceptor Tyrosine Kinases, Proline-Rich Tyrosine Kinase 2 and c-Src.
Salicylate Inhibits Phosphorylation of the Nonreceptor Tyrosine Kinases, Proline-Rich Tyrosine Kinase 2 and c-Src.
Schindler EM, Baumgartner M, Gribben EM, Li L, Efimova T
The Journal of investigative dermatology 2007 May;127(5):1094-106
The Journal of investigative dermatology 2007 May;127(5):1094-106
Role of PYK2 in the development of obesity and insulin resistance.
Yu Y, Ross SA, Halseth AE, Hollenbach PW, Hill RJ, Gulve EA, Bond BR
Biochemical and biophysical research communications 2005 Sep 9;334(4):1085-91
Biochemical and biophysical research communications 2005 Sep 9;334(4):1085-91
Heat shock protein 60 inhibits Th1-mediated hepatitis model via innate regulation of Th1/Th2 transcription factors and cytokines.
Zanin-Zhorov A, Bruck R, Tal G, Oren S, Aeed H, Hershkoviz R, Cohen IR, Lider O
Journal of immunology (Baltimore, Md. : 1950) 2005 Mar 15;174(6):3227-36
Journal of immunology (Baltimore, Md. : 1950) 2005 Mar 15;174(6):3227-36
Heat shock protein 60 inhibits Th1-mediated hepatitis model via innate regulation of Th1/Th2 transcription factors and cytokines.
Zanin-Zhorov A, Bruck R, Tal G, Oren S, Aeed H, Hershkoviz R, Cohen IR, Lider O
Journal of immunology (Baltimore, Md. : 1950) 2005 Mar 15;174(6):3227-36
Journal of immunology (Baltimore, Md. : 1950) 2005 Mar 15;174(6):3227-36
Implication of phospholipase D2 in oxidant-induced phosphoinositide 3-kinase signaling via Pyk2 activation in PC12 cells.
Banno Y, Ohguchi K, Matsumoto N, Koda M, Ueda M, Hara A, Dikic I, Nozawa Y
The Journal of biological chemistry 2005 Apr 22;280(16):16319-24
The Journal of biological chemistry 2005 Apr 22;280(16):16319-24
Glucose activates protein kinase C-zeta /lambda through proline-rich tyrosine kinase-2, extracellular signal-regulated kinase, and phospholipase D: a novel mechanism for activating glucose transporter translocation.
Bandyopadhyay G, Sajan MP, Kanoh Y, Standaert ML, Quon MJ, Reed BC, Dikic I, Farese RV
The Journal of biological chemistry 2001 Sep 21;276(38):35537-45
The Journal of biological chemistry 2001 Sep 21;276(38):35537-45
Salicylate Inhibits Phosphorylation of the Nonreceptor Tyrosine Kinases, Proline-Rich Tyrosine Kinase 2 and c-Src.
Wang Z, Brecher P
Hypertension (Dallas, Tex. : 1979) 2001 Jan;37(1):148-153
Hypertension (Dallas, Tex. : 1979) 2001 Jan;37(1):148-153
Salicylate Inhibits Phosphorylation of the Nonreceptor Tyrosine Kinases, Proline-Rich Tyrosine Kinase 2 and c-Src.
Wang Z, Brecher P
Hypertension (Dallas, Tex. : 1979) 2001 Jan;37(1):148-153
Hypertension (Dallas, Tex. : 1979) 2001 Jan;37(1):148-153
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of Phospho-FAK2 in extracts of pervanadate-treated primary chicken embryo fibroblasts plated on fibronectin, either mock-transfected (lane 1) or transfected with a Pyk2 expression vector (lanes 2-6). Results indicate specificity for Phospho-FAK2 Tyr881 (Product # 44-620G).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of PYK2 [pY881] was done on PC-3 cells treated with Pervanadate (100uM, 1 hour). Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with PYK2 [pY881] Rabbit Polyclonal Antibody (44620G, red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.
